Detection of lysozyme-like enzymatic activity secreted by an immune-responsive mosquito cell line

Nasr, Nadia ; Fallon, Ann 


Abstract


Using molecular approaches, we have recently shown that the C7-10 mosquito cell line from Aedes albopictus, and the Aag-2 line from Aedes aegypti, secrete a variety of immune peptides into the culture medium, including cecropins, defensins, transferrin, and lysozyme. The diversity of these peptides makes it difficult to quantify the relative activities of each molecule, because possible synergistic interactions may occur. Using a microtiter plate assay with live bacteria, we now show that C7-10 cells secrete an activity that is more potent against the Gram-positive bacterium, Micrococcus luteus, than against Gram-negative Escherichia coli. This lysozyme-like activity is accompanied by production of a lytic zone in an agarose plate assay containing commercially available, lyophilized M. luteus. Properties of the lysozyme-like activity from C7-10 cells included a broad pH optimum from 5.5 to 6.5, and heat-sensitivity above 42 C. Amounts of secreted activity increased during the initial 24 h of incubation with heat-killed bacteria. During this induction, lysozyme-like activity was found primarily in the cell culture supernatant. 2003 Elsevier Science (USA). All rights reserved.


Other data

Other Titles Detection of lysozyme-like enzymatic activity secreted by an immune-responsive mosquito cell line Article Apr 2003Journal of Invertebrate Pathology Nadia Mahmoud NasrAnn Fallon
Keywords immunity, Mosquito, Lys0zyme:;Insect immunity; Aedes albopictus; Mosquito; Cell line; Antibacterial activities
Issue Date Apr-2003
Publisher Seince Direct, Elsevier
Source 9
Journal Journal of Invertebrate Pathology 
Conference the conference of American Society of Entomology. Colorado, USA 
Description This work was supported by AI 36258 from the US National Institutes of Health and by the University of Minnesota Agricultural Experiment Station, St. Paul, MN. Dr. Nasr was supported by a fellowship from the Cultural and Educational Bureau of Egypt. W
URI http://research.asu.edu.eg/handle/123456789/2418


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