Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma
Tarek, M.; Louka, M.L.; Khairy, E.; Ali-Labib, R.; Zaky, D.Z.; Montasser, I.F.;
Abstract
There is an obvious need to diagnose hepatocellular carcinoma using novel non-invasive and sensitive biomarkers. In this regard, the aim of this study was to evaluate and correlate both relative quantification of microRNA-7 using quantitative real time polymerase chain reaction and quantitative analysis of selenoprotein P using enzyme-linked immunosorbent assay in sera of hepatocellular carcinoma patients, chronic liver disease patients, as well as normal healthy subjects in order to establish a new diagnostic biomarker with a valid non-invasive technique. In addition, this study aimed to investigate whether changes in selenium supply affect microRNA-7 expression and selenoprotein P levels in human hepatocarcinoma cell line (HepG2). The results showed a highly significant decrease in serum microRNA-7 relative quantification values and selenoprotein P levels in malignant group in comparison with benign and control groups. The best cutoff for serum microRNA-7 and selenoprotein P to discriminate hepatocellular carcinoma group from benign and control groups was 0.06 and 4.30 mg/L, respectively. Furthermore, this study showed that changes in selenium supply to HepG2 cell line can alter the microRNA-7 profile and are paralleled by changes in the concentration of its target protein (selenoprotein P). Hence, serum microRNA-7 and selenoprotein P appear to be potential non-invasive diagnostic markers for hepatocellular carcinoma. Moreover, the results suggest that selenium could be used as an anticancer therapy for hepatocellular carcinoma by affecting both microRNA-7 and selenoprotein P.
Other data
Title | Role of microRNA-7 and selenoprotein P in hepatocellular carcinoma | Authors | Tarek, M. ; Louka, M.L. ; Khairy, E. ; Ali-Labib, R. ; Zaky, D.Z. ; Montasser, I.F. | Issue Date | 2017 | Journal | Tumor Biology | DOI | 5 1010428317698372 http://www.scopus.com/inward/record.url?eid=2-s2.0-85019984252&partnerID=MN8TOARS 39 1423-0380 10.1177/1010428317698372 |
PubMed ID | 28459371 | Scopus ID | 2-s2.0-85019984252 |
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