THE PROTECTIVE ROLE OF "CLEOME DROSERIFOLIA" EXTRACT AGAINST TESTICULAR TOXICITY INDUCED BY LAMBDA-CYHALOTHRIN IN RATS

Abstract

Lambda-cyhalothrin (LCT) is pyrethroid insecticide that is used worldwide for pest control in agriculture and household use. Cleome droserifolia (CDE) extract is a potential antioxidant protecting cells from oxidative stress. The present study investigates the protective effect of CDE on LCT-induced changes in male rats. Forty adult male albino rats were divided into four groups: group I: served as control; group II: received LCT (6.23 mg/kg b.w.,i.p.) only; group III: received CDE (100 mg/kg b.w.,p.o.) only; group IV: received both CDE and LCT. LCT-intoxication elicited significant declines in serum testosterone, total protein and albumin, high density lipoprotein (HDL), whereas, serum follicle stimulating hormone (FSH) and luteinizing hormone (LH), total cholesterol, triglycerides (TG), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) levels were significantly increased. Furthermore, LCT brought about significant disturbance in cytokines; tumor necrosis factor (TNF-α), interleukin-10(IL-10) andinterleukin-12(IL-12) levels. Testicular glutathione (GSH) content and superoxide dismutase (SOD) activity reduced significantly, while, testicular malondialdehyde (MDA) level was significantly elevated after LCT administration as compared to the control group. Histological and ultrastructural changes were also in accord with the above aberrations. However, treatment with CDE significantly attenuated LCT-induced changes in clinical chemistry parameters as well as it protected testicular tissues and cell structures. In conclusion, CDE has a potential protective role in ameliorating testicular toxicity induced by LCT in albino rats.