Role of Mesenchymal Stem Cells in the Treatment of Testicular Toxicity Induced by Lambda-Cyhalothrin in Rats

Abstract

The present study aimed to investigate the therapeutic effects of MSCs on lambda-cyhalothrin (LCT)-induced testicular toxicity in rats. Thirty adult male rats were divided into three groups: group I: normal control; group II: received LCT (1/10DL50 = 6.23 mg/kg, intraperitoneally (i.p.)); group III: received LCT (6.23 mg/kg)+MSCs(1x106 cells/animal, intravenously (i.v.)). LCT elicited significant (p<0.001) declines in serum testosterone, proteins profile, testicular glutathione (GSH) and superoxide dismutase (SOD) activity and the anti-inflammatory cytokine; interleukin-10 (IL-10), whereas, serum follicle stimulating hormone (FSH), luteinizing hormone (LH), lipid profile and pro-inflammatory cytokines, tumor necrosis factor (TNF-?) and interleukin-12 (IL-12) levels were significantly (p<0.001) increased as compared to the control group. Light microscopic examination showed abnormal germ cells displaying nuclear pyknosis or karyolysis. Lacking of both spermatogenic cells and mature spermatozoa, hyalinization of the seminiferous tubules and congestion of the blood vessels were also observed. Ultrastructure changes confirmed the light microscope results. Compared to LCT-rats, LCT+MSCs-treated group showed significant improvement in the activity of all biochemical parameters and marked preservation in histological and ultrastructural configuration of the testis. It can be concluded that therapy with MSCs was effective in ameliorating LCT-induced testicular damage by improvement of testicular function and structure as well as male fertility.