EFFECTS OF SOME ANTIOXIDANT VITAMINS AND CHELATING AGENT ON BIOCHEMICAL ALTERATIONS IN HYPERCHOLESTEROLEMIC RATS.

Abstract

A total number of sixty adult male Wistar rats were used in this study to investigate the protective effects of vitamin C (Vit C),Vit E and ethylenediaminetetraacetic acid (EDTA) alone and their combinations on hypercholesterolemia through markers of lipid profile, thyroid function, some liver and oxidative enzymes. Rats were randomly divided into six groups (n = 10). Group I: normal control, Group II: hypercholesterolemic rats, Groups III: received EDTA (1gEDTA/kg b.wt.), group IV: received 1g/Kg b.wt. of Vit C, group V: received Vit E1g/Kg feed and Group VI: received EDTA, Vit C and Vit E. The results of this study showed that ration supplemented with EDTA, VC and Vit E either individual or in a Combination group induced significant decrease (P<0.05) in total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) levels in hypercholesterolemic rats. Also, atherogenic index (AI), lipid peroxidation(MDA), thyroid stimulating hormone (TSH), the activities of serum aspartate and alanine aminotransferases (AST, ALT), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT) levels recorded significant decrease, but high-density lipoprotein (HDL), superoxide dismutase (SOD), reduced glutathione(GSH), serum triiodothyronine (T3) and thyroxine (T4) exhibited significant increase. Moreover, the administration of EDTA to hypercholesterolemic rats (group III) causes significant decrease in serum calcium (Ca), phosphorus (P), magnesium (Mg) and iron with significant increase in total iron binding capacity (TIBC) in comparison with other groups. The overall result, the improvement being more in rats treated with mixture of EDTA, Vit C and Vit E The present results suggested that supplementation with EDTA, Vit C and Vit E attenuated most of the changes induced in hypercholesterolemic rats by feeding rats with cholesterol-rich diet owing to their observed anti-hyperlipidemic and antioxidant properties. In conclusion, the present study provides obvious evidence that both Vit C, Vit E and EDTA hold the promise of being useful hypocholesterolemic, and peroxidative agent and should be administered to the body continuously, targeted to the biological site susceptible to oxidative damage.