Protective effect of grape seeds on some antioxidant parameters and hormonal changes in female rats intoxicated with methoxychlor insecticide

Abstract

The present study was designed to establish the protective role of grape seeds proanthocyanidins(GSP) on methoxychlor (MXC) induced oxidative stress and the resultant dysfunction in female rats ovary. The study was performed using forty adult female rats. They were allocated into four groups: 1st group served as a control group. 2nd group received MXC (200 mg/kg b.w. twice / week, orally) for 28 days. 3rd group, received GSP (100 mg/kg b.w. orally) daily for 28 days. 4th group received both GSP plus MXC in the same previous doses and routes. At the end of the experiment, all animals were sacrificed and serum samples were collected for some antioxidant and hormonal analysis. The results revealed that administration of MXC for 28 days induced a significant increase in serum levels of malondialdehyde (MDA) as a marker of lipid peroxidation, and nitric oxide (NO) and also, a significant decrease in serum levels of reduced glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GPx). In addition (MXC) treated group induced a significant decrease in levels of follicle stimulating (FSH) hormone, Luteinizing (LH) hormone, estrogen and progesterone while prolactin hormone was significantly increased. On the other hand, administration of GSP plus MXC improved significantly the previous antioxidant and hormonal parameters comparing with (MXC) treated group. It's concluded that co-treatment with grape seeds proanthocyanidins(GSP) could ameliorate the toxicity induced by MXC, which ensure the protective effect of GSP.