Celery oil modulates DEHP-induced reproductive toxicity in male rats
Helal, M.A.M.; Helal, M.A.M.;
Abstract
The objective of the study was to investigate the protective effect of Apium graveolens (AP) against di-(2-ethylhexyl) phthalate (DEHP)-induced testes injury in rats. Adult rats were divided into nine groups: (1) control group (no treatment); (2) corn oil (60. μg/kg body weight - bwt); (3) AP (50. μg/kg bwt); (4) 300. mg DEHP/kg bwt; (5) 500. mg DEHP/kg bwt; (6) 1000. mg DEHP/kg bwt; (7) 300. mg DEHP/kg bwt + AP; (8) 500. mg DEHP/kg bwt + AP; and (9) 1000. mg DEHP/kg bwt + AP. Oral administration of treatments was performed daily for 6 weeks. DEHP decreased (p < 0.01) body weight, testis weight and serum concentrations of testosterone, cholesterol and total proteins. Moreover, DEHP increased (p < 0.001) total antioxidant capacity in the testis and plasma DEHP level. In addition, DEHP decreased mRNA expression of two testicular steroidogenic enzymes: 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase. DEHP also caused atrophy, vacuolar degeneration and aspermia of the seminiferous tubules. AP administered concurrently with DEHP effectively alleviated most of the DEHP-induced effects. In conclusion, in male rats, DEHP had adverse effects on the testis including inhibition of androgen production. A concurrent administration of A. graveolens (celery oil) protected the testis against DEHP-induced toxicity. © 2014 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn.
Other data
Title | Celery oil modulates DEHP-induced reproductive toxicity in male rats | Authors | Helal, M.A.M. ; Helal, M.A.M. | Issue Date | 1-Jan-2014 | Journal | Reproductive Biology | DOI | 3 182 http://api.elsevier.com/content/abstract/scopus_id/84906514309 14 2300-732X 10.1016/j.repbio.2014.04.002 |
PubMed ID | 14 | Scopus ID | 2-s2.0-84906514309 |
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