Biochemical studies on the effect of Chitin synthesis inhibitor, (flufenoxuron) and SpliNPV on the cotton leaf worm Spodoptera littoralis Bosid (Lepidoptera:Noctuidae)

Abstract

In this studyflufenoxuron as insect growth regualtor and SpliNPV were used to investigate their toxicity on Spodopteralittoralis. Both 2nd and 4th larval instars of S. littoralis were exposed to the different concentrations of flufenoxuron and SpliNPV. The biochemical effect of IGR and SpliNPV on both 2nd and 4th larvae instar show changes in protein electrophoretic pattern in the current study reveals differences between the untreated samples and treated one, some proteins were missed or expressed at different stages which may be responsible for all the obtained deformation. The effect of these compounds on biochemical activities were revealed after studying electrophoretic protein (fractionated protein) using PAGE in order to determine their effects on the vital system of S. littoralis, which may indicate the formation of new proteins responsible for stimulating the immune system of the insect as a result of entering foreign objects inside the body of the insect.