RAPD-PCR and protein electrophoresis of Monomorium pharaoni worker after IGR's treatments

Abstract

The present study examined the effect of certain IGRs (pyriproxyfen & chlorfluazuron) on molecular criteria of the Pharaoh ant workers Monomorium pharaonis (L.). Different concentrations of 0.1%, 0.5% & 1% pyriproxyfen and 0.001%, 0.01% & 0.1% chlorfluazuron were used. The study revealed that, the most effective IGRs was chlorfluazuron. The total protein was stimulated by IGRs. A high genetic distance (0.66 and 0.65) was recorded using electrophoretic protein between the control and treated samples by pyriproxyfen and chlorfluazuron respectively. By using RAPD-PCR technique, four random primers screened workers DNA and generated 104 fragments ranged from 132 to 1059 bp. The highest genetic distance (1) was recorded between control sample and treated sample by 1% pyriproxyfen by using primer OPA-7.