Methyl Farnesoate Plays a Dual Role in Regulating Drosophila Metamorphosis

Wen D, Rivera-Perez C, Abdou M, Jia Q, He Q, Liu X, Zyaan O, Xu J, Bendena WG, Tobe SS, Noriega FG, Palli SR, Wang J, Li S. ; Abdou, Mohamed 


Abstract


© 2015 Wen et al. Corpus allatum (CA) ablation results in juvenile hormone (JH) deficiency and pupal lethality in Drosophila. The fly CA produces and releases three sesquiterpenoid hormones: JH III bisepoxide (JHB3), JH III, and methyl farnesoate (MF). In the whole body extracts, MF is the most abundant sesquiterpenoid, followed by JHB3 and JH III. Knockout of JH acid methyl transferase (jhamt) did not result in lethality; it decreased biosynthesis of JHB3, but MF biosynthesis was not affected. RNAi-mediated reduction of 3-hydroxy-3-methylglutaryl CoA reductase (hmgcr) expression in the CA decreased biosynthesis and titers of the three sesquiterpenoids, resulting in partial lethality. Reducing hmgcr expression in the CA of the jhamt mutant further decreased MF titer to a very low level, and caused complete lethality. JH III, JHB3, and MF function through Met and Gce, the two JH receptors, and induce expression of Kr-h1, a JH primary-response gene. As well, a portion of MF is converted to JHB3 in the hemolymph or peripheral tissues. Topical application of JHB3, JH III, or MF precluded lethality in JH-deficient animals, but not in the Met gce double mutant. Taken together, these experiments show that MF is produced by the larval CA and released into the hemolymph, from where it exerts its anti-metamorphic effects indirectly after conversion to JHB3, as well as acting as a hormone itself through the two JH receptors, Met and Gce.


Other data

Issue Date 16-Mar-2015
Journal PLoS Genetics 
URI http://research.asu.edu.eg/123456789/791
DOI 3
e1005038
http://api.elsevier.com/content/abstract/scopus_id/84926167999
11
1553-7404
10.1371/journal.pgen.1005038


Recommend this item

CORE Recommender
29
Citations

17
Views


Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.