The proliferative response of T cells to Aspergillus antigen requires prior presentation on dendritic cells

Ramadan, Gamal 


Abstract


Dendritic cells (DC) are the most powerful antigen presenting cells that induce and maintain primary immune response. The present study confirms and extends the evidence that the proliferative response of T lymphocytes to Aspergillus antigen requires prior presentation on DC. Adherent blood monocytes (Mo) from healthy subjects were treated for 5 days with GM-CSF and IL-4 to generate immature DC and with IL-1β, IL-6, TNF-α and PGE2 for 2 days to generate matured DC and pulsed with Aspergillus antigen immediately prior to maturation. The Aspergillus-pulsed DC demonstrated the typical morphology, cytoplasmic projections and surface markers of mature DC. Lymphocyte proliferation in response to soluble Aspergillus crude extract was compared with that presented by ex vivo generated autologous DC. The Aspergillus crude extract demonstrated marked toxic effect on peripheral blood mononuclear cells (PBMC) at concentrations started from 25 μg/ml and failed to initiate lymphocyte proliferative response. In contrast Aspergillusspecific T cell proliferation was demonstrated when cocultured with autologous Aspergillus-pulsed, Moderived DC and the culture supernatant showed marked IFN-γ secretion. In conclusion, this study pointed out that the proliferative Th1 response to Aspergillus requires prior presentation on DC.


Other data

Issue Date 2004
Publisher The Egyptian Association of Immunologists
Journal The Egyptian Journal of Immunology 
URI http://research.asu.edu.eg/123456789/799
DOI http://www.scopus.com/inward/record.url?eid=2-s2.0-33750715772&partnerID=MN8TOARS
http://www.scopus.com/inward/record.url?eid=2-s2.0-33750715772&partnerID=MN8TOARS
http://www.scopus.com/inward/record.url?eid=2-s2.0-33750715772&partnerID=MN8TOARS


Recommend this item

CORE Recommender
3
Citations

4
Views


Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.