Fast monocyte-derived dendritic cell-based immunotherapyRamadan, Gamal
Abstract© Springer Science+Business Media New York 2014. Recent reports have described a new strategy for differentiation and maturation of monocyte (Mo)-derived dendritic cells (DC) within only 48-72 h of in vitro culture (fast-DC). Mature fast-DC are as effective as mature standard-DC (generated in 7-10 days of in vitro culture) in priming and propagation of antigenspecific T-cell responses. The use of fast-DC not only reduces labor and supply cost, as well as workload and time, but also increases the DC yield from Mo, which may facilitate DC-based immunotherapy for cancer patients. Detailed protocols for generation, pulsing with different antigen sources, and transduction with adenoviral vector of Mo-derived mature fast-DC as well as using of fast-DC for priming and propagation of antigen-specifi c cytotoxic T-cell effectors will be described here.
|Issue Date||1-Jan-2014||Journal||Methods in Molecular Biology||URI||http://research.asu.edu.eg/123456789/805||DOI||131
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