Fast monocyte-derived dendritic cell-based immunotherapy

Ramadan, Gamal 


Abstract


© Springer Science+Business Media New York 2014. Recent reports have described a new strategy for differentiation and maturation of monocyte (Mo)-derived dendritic cells (DC) within only 48-72 h of in vitro culture (fast-DC). Mature fast-DC are as effective as mature standard-DC (generated in 7-10 days of in vitro culture) in priming and propagation of antigenspecific T-cell responses. The use of fast-DC not only reduces labor and supply cost, as well as workload and time, but also increases the DC yield from Mo, which may facilitate DC-based immunotherapy for cancer patients. Detailed protocols for generation, pulsing with different antigen sources, and transduction with adenoviral vector of Mo-derived mature fast-DC as well as using of fast-DC for priming and propagation of antigen-specifi c cytotoxic T-cell effectors will be described here.


Other data

Issue Date 1-Jan-2014
Journal Methods in Molecular Biology 
URI http://research.asu.edu.eg/123456789/805
DOI 131
http://api.elsevier.com/content/abstract/scopus_id/84909580806
1139
1940-6029
10.1007/978-1-4939-0345-0_12


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