Clinical significance of high levels of survivin and transforming growth factor beta-1 proteins in aqueous humor and serum of retinoblastoma patients

Shehata HH ; Elsayed EK ; Azza Mohamed Ahmed Said ; Mahmoud SS 


Abstract


© 2016 American Association for Pediatric Ophthalmology and Strabismus Purpose To evaluate the diagnostic and prognostic values of survivin and transforming growth factor beta-1 (TGF-B1) expression in aqueous humor and serum of retinoblastoma (RB) in comparison to the conventional RB marker lactate dehydrogenase (LDH) and to elucidate a possible correlation between them and the clinicopathological features of the disease. Methods This prospective, comparative study included 88 newly diagnosed children with RB and 80 age-matched controls with ophthalmic conditions other than tumors prepared for intraocular surgeries. Concentrations of survivin, TGF-B1, and LDH were measured in serum and aqueous humor before and 6 months after completion of therapy. Results High serum and aqueous humor concentrations of the three pro teins were detected in RB patients before treatment compared to the control group (P  <  0.01), with a significant reduction of serum concentrations after treatment (P  <  0.01). For the highest sensitivity and specificity, the optimal cutoff values of serum and aqueous survivin were 12.9 pg/ml and 25.2 pg/mg, with a significant positive correlation between aqueous survivin and RB staging and presence of optic nerve infiltration (r = 0.43, P = 0.04); the best cutoff values of serum and aqueous TGF-B1, 370.7 pg/ml and 39.8 pg/mg, with a significant positive correlation between aqueous TGF-B1 and poor differentiation of the tumor (r = 0.69, P = 0.001). Conclusions The high sensitivity, specificity, and accuracy of serum and aqueous humor survivin and TGF-B1 proteins make them promising markers for early detection and follow-up of RB patients.


Other data

Issue Date 1-Oct-2016
Journal Journal of AAPOS 
URI http://research.asu.edu.eg/123456789/95
DOI 5
444.e1
http://api.elsevier.com/content/abstract/scopus_id/84994899457
20
1528-3933
10.1016/j.jaapos.2016.07.223


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