HPLC-PDA-ESI-MS/MS profiling and chemopreventive potential of Eucalyptus gomphocephala DC

Al-Sayed E. ; Singab A. ; Ayoub N. ; Martiskainen O. ; Sinkkonen J. ; Pihlaja K. 


Abstract


An HPLC-PDA-ESI/MS/MS method was developed to identify the phytoconstituents of the EtOAc fraction of Eucalyptus gomphocephala DC. The antioxidant effect of the EtOAc fraction together with its sub-fractions was determined in vitro. The cytotoxicity was evaluated on different cell lines. The EtOAc fraction exhibited strong antioxidant activity, reduced the viability of all cell lines and was more active on MCF-7 and HepG-2 cell lines. Subsequently, the cytotoxicity of the sub-fractions and the isolated compounds were tested on MCF-7, HepG-2. The EtOAc fraction possessed potential antitumour promoting properties. It inhibited the stimulated NO (20%), 5-LOX (48.0%) and COX-2 (49.7%) respectively (at concentration of 20 μg/ml). This study suggests that this fraction is a source of different antioxidant and cytotoxic compounds with potential chemopreventive properties that might prevent different stages of the carcinogenesis process. © 2012 Elsevier Ltd. All rights reserved.


Other data

Issue Date 1-Aug-2012
Journal Food Chemistry 
URI http://research.asu.edu.eg/123456789/968
DOI 3
1017
http://api.elsevier.com/content/abstract/scopus_id/84858337787
133
10.1016/j.foodchem.2011.09.036


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