http://hdl.handle.net/123456789/2 2026-06-02T06:04:39Z http://hdl.handle.net/123456789/227879 Title: Novel method for determination of anthracene by coupling dispersive liquid-liquid extraction to first-derivative synchronous spectrofluorimetry Authors: Abdel-Aziz, Omar; El Kosasy, A. M.; Okeil, Sherif Abstract: A novel method could be adopted successfully for determination of anthracene in environmental samples, utilizing dispersive liquid-liquid extraction followed by first-derivative synchronous fluorimetry at a constant wavelength difference Δλ=165 nm, where a linear calibration curve was obtained in a concentration range of 0.5-100 ng mL^-1 at 244 nm. The detection limit was 0.1 ng mL^-1. The method can be easily adopted for determination of anthracene in aqueous media including tap water and river water. The recoveries obtained were 85.40-108.02 %. The proposed method was validated according to International Conference of Harmonization (ICH) guide lines and successfully applied to determine anthracene in pure form and in water samples including real life water samples from different sources. All the results obtained were compared with those of published method, where no a significant difference was observed. © 2014 Springer Science+Business Media New York. 2014-01-01T00:00:00Z http://hdl.handle.net/123456789/227700 Title: First fluorescence method for monitoring the doping stimulant drug solriamfetol in plasma and urine: A pharmacokinetic study in volunteers’ urine with greenness, whiteness, and blueness assessments Authors: Kamel, Marina Z.; Hussein, Lobna. A.; Trabik, Yossra; Yamani, Hend. Z.; Yamani, Hend Z. Abstract: A green, sustainable, simple, and highly sensitive spectrofluorimetric method was developed to determine solriamfetol hydrochloride for the first time in biological fluids and dosage form. To the best of our knowledge, no Fluorimetric methods have been reported for solriamfetol determination in plasma and urine. The method is based on measuring the intrinsic fluorescence of solriamfetol hydrochloride at 522 nm after excitation at 260 nm in ammonium acetate buffer solution at pH 4. All factors influencing the response have been carefully investigated. The method was linear over a concentration range of 10 to 1800 ng/mL (r = 0.9998). The limit of detection (LOD) and limit of quantification (LOQ) values were found to be 3.2 and 9.8 ng/mL, respectively, indicating the excellent sensitivity of the proposed method. The pharmaceutical dosage form, spiked human plasma, and urine samples were successfully analyzed using the described method with average recoveries of 99.79 ± 0.739, 99.27 ± 1.885, and 99.57 ± 1.701, respectively. Plasma samples were first subjected to protein precipitation using 10 % perchloric acid, while urine samples were analyzed directly without any pretreatment. Furthermore, the suggested method was applied effectively on real human volunteers’ urine to measure renal clearance, the cumulative amount of solriamfetol excreted unchanged in the urine, and the percent of the dose recovered. The eco-friendly nature and sustainability of this work were evaluated using trending greenness, whiteness, and blueness assessment metrics. The greenness assessment tools, namely the Analytical GREEnness metric tool (AGREE), Analytical Eco-scale, and complementary green analytical procedure index (complex GAPI) were selected to prove the eco-friendly nature of the method. Additionally, whiteness and blueness assessments were conducted using the Red-Green-Blue model (RGB model), and high Blue Applicability Grade Index (BAGI), respectively. The proposed method is a promising analytical tool for routine analysis in quality control labs, therapeutic drug monitoring, and pharmacokinetics studies. 2024-12-01T00:00:00Z http://hdl.handle.net/123456789/227656 Title: Photodynamic drug delivery for cancer therapy: Designing liposomes for light-controlled release and enhanced drug efficacy Authors: Tristan Le Clainche; ABDELHAMID, Ahmed Gamal Ali; Nazareth Milagros Carigga Gutierrez; Marie-Anne Jourdain; Sofia Leo; Lucie Sancey; Amandine Hurbin; Jean-Luc Coll; Bénédicte Elena-Herrmann; Mans Broekgaarden Abstract: Photodynamics involves the use of photocatalytic compounds that, upon excitation with light, produce reactive oxygen species and have seen widespread applications in the treatment of cancer in the form of photodynamic therapy. Within the field of drug delivery, photodynamics has emerged as a strikingly effective approach for spatiotemporal-controlled drug release by harnessing photochemical redox reactions to destabilize lipid nanoformulations that contain oxidation-susceptible excipients. Despite highly promising outcomes in preclinical models, such controlled release modalities have not yet been explored in clinical cancer trials. This review outlines key design considerations for lipid nanoformulations in photodynamic drug delivery, focusing on their susceptibility to photochemical redox reactions, their ability to induce lysosomal permeabilization, and facilitate microenvironmental priming that enhances tumor permeability. These considerations first highlight the role of specific lipid excipients in determining photodynamic drug release efficiencies. Secondly, the selection of the photosensitizing agents is considered, which ideally absorb light >650 nm and exhibit limited leaching. Thirdly, the selected photosensitizing agent and pharmaceutical cargoes may dictate which drug loading approach should be pursued, and how drug release is detected. We particularly highlight the promise of nuclear magnetic resonance (NMR) spectroscopy as it can provide non-destructive quantification of encapsulated and released pharmaceutical cargoes, alongside structural assessments of the lipid nanoformulations, without the need for prior separation or complex sample preparation. Finally, considering the corollary effects of photodynamics on cancer cells and the cancer microenvironment, we emphasize the utility of a multi-model approach to evaluate novel photodynamic drug delivery systems. By providing these design considerations, this review aims to boost the field of photodynamic drug delivery and encourage its exploitation in translational cancer research. Moreover, these aspects are equally relevant to stimulate investigations towards oxidation-responsive drug release triggered by alternative external stimuli, thereby broadening the drug delivery arsenal against cancer. 2025-01-01T00:00:00Z http://hdl.handle.net/123456789/227655 Title: Applying photodynamics for radiotherapy-controlled drug delivery Authors: Nazareth M. Carigga Gutierrez; Amandine Hurbin; Anne-Laure Bulin; Jean-Luc Coll; Mans Broekgaarden; Ahmed Gamal Ali ABDELHAMID Abstract: Liposomes have been the most successful type of nanomedicine for cancer patients, playing a leading role in improving the tolerability of chemotherapeutics. However, to advance the success of liposomal drug delivery and cancer treatment, new approaches to physically trigger drug release in cancer tissues and increase the permeability of the protective cancer stroma are needed. To this end, photodynamics has been successfully harnessed for spatiotemporal-controlled drug release, alleviation of cancer desmoplasia, and improved efficacy of co-encapsulated or adjuvant chemotherapeutics. In this work, we present the adaptation of this approach for radiotherapy-controlled drug release, by functionalizing these liposomes with radiocatalytic- high-Z element-based nanomaterials. This strategy was explored in the context of chemo-radiation therapy for pancreatic cancer using in silico modeling, in vitro microtumor cultures, and orthotopic in vivo models of pancreatic cancer. 2025-01-01T00:00:00Z