Development of an intracanal polymicrobial biofilm and its susceptibility to different antibacterial intracanal medications .an in vitro study
Nada Ahmed Zakaria Mohamed Al Shelk;
Abstract
The aim of this study was to develop an intracanal polymicrobial biofilm and testing its susceptibility to different antibacterial medications in vitro
A total of seventy single rooted human teeth were used in this study .Teeth were decoronated to a length of 16 mm, cleaned and shaped , longitudinal grooves were made along the entire length of the teeth before preparation and the teeth were divided into 9 groups , 7 experimental groups (n=8) and 2 control groups ,the positive control group (n=10) and the negative control group (n=4).The positive control group and the experimental groups were incubated with previously cultured Enterococcus faecalis,Staphylococcus epidermidis and Candida albicans to form a multispecies biofilm for 3 weeks at 37°C incubator.
Six samples were scanned for evaluating the biofilm development by SEM examination at three time intervals (days 7,14,21).
The 3-week-old bacterial biofilm grown on samples was observed for thickness, homogeneity, and presence of an extracellular polymeric substance using scanning electron microscopy.
After 21 days of incubation, calculated MICs of antimicrobials tested were applied to the experimental groups.
Group 1: received 0.5 ml of 0.04 mg ciprofloxacin.
Group 2: received 0.5 ml of 10 mg Doxycycline.
Group 3: received 0.5ml of 30 mg Clindamycin.
Group 4: received 0.5 ml of 0.1 mg of metronidazole.
Group 5: received 0.5 ml of 24 mg Augmentin.
Group 6: received 0.5 ml of 300 mg triple antibiotic paste, consisting of equal ratios of (Doxycycline, Ciprofloaxcin and metronidazole).
Group 7 received 0.5 ml of calcium hydroxide.
After one week, all samples were irrigated with 20 ml sterile saline solution to remove the root canal contents, bacterial samples were taken using a standard method of collection and collected in sterile tubes containing saline for CFU, the effect of tested drugs on the biofilm dissolution was assessed with scanning electron microscope.
Results showed that all the experimental groups demonstrated significantly less CFU counts as compared to the
A total of seventy single rooted human teeth were used in this study .Teeth were decoronated to a length of 16 mm, cleaned and shaped , longitudinal grooves were made along the entire length of the teeth before preparation and the teeth were divided into 9 groups , 7 experimental groups (n=8) and 2 control groups ,the positive control group (n=10) and the negative control group (n=4).The positive control group and the experimental groups were incubated with previously cultured Enterococcus faecalis,Staphylococcus epidermidis and Candida albicans to form a multispecies biofilm for 3 weeks at 37°C incubator.
Six samples were scanned for evaluating the biofilm development by SEM examination at three time intervals (days 7,14,21).
The 3-week-old bacterial biofilm grown on samples was observed for thickness, homogeneity, and presence of an extracellular polymeric substance using scanning electron microscopy.
After 21 days of incubation, calculated MICs of antimicrobials tested were applied to the experimental groups.
Group 1: received 0.5 ml of 0.04 mg ciprofloxacin.
Group 2: received 0.5 ml of 10 mg Doxycycline.
Group 3: received 0.5ml of 30 mg Clindamycin.
Group 4: received 0.5 ml of 0.1 mg of metronidazole.
Group 5: received 0.5 ml of 24 mg Augmentin.
Group 6: received 0.5 ml of 300 mg triple antibiotic paste, consisting of equal ratios of (Doxycycline, Ciprofloaxcin and metronidazole).
Group 7 received 0.5 ml of calcium hydroxide.
After one week, all samples were irrigated with 20 ml sterile saline solution to remove the root canal contents, bacterial samples were taken using a standard method of collection and collected in sterile tubes containing saline for CFU, the effect of tested drugs on the biofilm dissolution was assessed with scanning electron microscope.
Results showed that all the experimental groups demonstrated significantly less CFU counts as compared to the
Other data
| Title | Development of an intracanal polymicrobial biofilm and its susceptibility to different antibacterial intracanal medications .an in vitro study | Other Titles | تكوين فيلم حيوي متعدد الميكروبات داخل القنوات العصبية واختبار المقاومة البكتيرية لأنواع مختلفة من مضادات البكتيريا المستخدمة كعقار للقنوات العصبية | Authors | Nada Ahmed Zakaria Mohamed Al Shelk | Issue Date | 2016 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| G11429.pdf | 777.71 kB | Adobe PDF | View/Open |
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