Biochemical study of some hormonal changes in polycystic ovarian patients

Abstract

This study aimed to determine the potential usefulness of serum melatonin (Mel), urinary 6-sulfatoxymelatonin (aMT6s), serum thyroid stimulating hormone (TSH), serum follicle stimulating hormone (FSH), serum luteinizing hormone (LH), serum prolactin (PRL), serum estradiol (E2), serum progesterone, serum sex hormone binding globulin (SHBG), serum free testosterone and serum dehydroepiandrosterone sulfate (DHEAS) as diagnostic and/ or prognostic markers in women with polycystic ovary syndrome (PCOS). This study included 30 women with PCOS, 15 perimenopausal women, 15 postmenopausal women and 30 healthy women. Mel, aMT6s and SHBG were determined by enzyme linked immunosorbent assay (ELISA), free testosterone and DHEAS were measured by radioimmunoassay technique (RIA) while TSH, FSH, LH, PRL, E2 and progesterone were measured by chemiluminescent immunoassay. Our results showed that the mean values of Mel night (N), Mel day (D), aMT6s, FSH, LH, free testosterone, PRL and TSH were significantly increased while SHBG, E2 and progesterone were significantly decreased in PCOS as compared with controls. The mean values of FSH, LH and free testosterone were significantly increased while the mean values of MEL- N, aMT6s, DHEAS, SHBG, E2 and progesterone were significantly decreased in perimenopausal and postmenopausal women as compared with controls. PRL and TSH were significantly increased in postmenopausal women while Mel-D was significantly decreased in postmenopausal women as compared with controls. Mel-N, Mel-D, aMT6s, progesterone, free testosterone, TSH, LH, PRL and DHEAS were significantly increased while FSH and PRL significantly decreased in PCOS as compared with perimenopause women. MEL-N, MEL-D, aMT6s, progesterone, SHBG, E2 and DHEAS were significantly increased while FSH and LH significantly decreased in PCOS as compared with postmenopausal women. Mel-D, E2, progesterone, SHBG and DHEAS were significantly increased while free testosterone, TSH, PRL, FSH, LH were significantly decreased in perimenopausal women as compared with postmenopausal