MOLECULAR DETECTION OF ISONIAZID AND RIFAMPICIN RESISTANT MYCOBACTERIUM TUBERCULOSIS
Hadir Ahmed Said Okasha;
Abstract
Presently, tuberculosis (TB) reemergence and spread are of worldwide concern. The situation is aggravated by the increasing circulation of multidrug resistant (MDR) Mycobacterium tuberculosis strains that are defmed as resistant to at least rifampicin (RJF) and isoniazid (INH), which comprise the back-bone of antitubercular chemotherapy.
INH resistance is apparently controlled by a more complex genetic system that involves several genes, namely, katG, inhA, kasA, and ahpC . Finally, extensive studies have demonstrated that INH resistance is most frequently associated with mutations in katG, codon 315.
In M.tuberculosis resistance to rifampicin almost invariably involves alterations of RNA polymerase, which is encoded by the RNA polymerase subunit gene (rpoB), mutations of this gene subunit was found to be responsible for rifampicin resistance in more than 95% of rifampicin resistant M. tuberculosis.
The aim of the present study was to test a simple and rapid PCR assay for detecting INH and RIF resistance in M. tuberculosis clinical isolates.
INH resistance is apparently controlled by a more complex genetic system that involves several genes, namely, katG, inhA, kasA, and ahpC . Finally, extensive studies have demonstrated that INH resistance is most frequently associated with mutations in katG, codon 315.
In M.tuberculosis resistance to rifampicin almost invariably involves alterations of RNA polymerase, which is encoded by the RNA polymerase subunit gene (rpoB), mutations of this gene subunit was found to be responsible for rifampicin resistance in more than 95% of rifampicin resistant M. tuberculosis.
The aim of the present study was to test a simple and rapid PCR assay for detecting INH and RIF resistance in M. tuberculosis clinical isolates.
Other data
| Title | MOLECULAR DETECTION OF ISONIAZID AND RIFAMPICIN RESISTANT MYCOBACTERIUM TUBERCULOSIS | Other Titles | الكشف الجزيئى لميكروب الدرن المقاوم للايزونيازيدوالريفمبيسين | Authors | Hadir Ahmed Said Okasha | Issue Date | 2005 |
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