EXPRESSION OF THE MOSQUITOCIDAL TOXIN GENE OF BAC/llUSTBU/l/NGIENSIS(H•14) IN CERTAIN SOIL BACTERIA, FOR MICROBIAL CONTROL OF MOSQUITO LARVAE

Abstract

The planned work and the results achieved during the present work can be summarized as follows: The work plan is divided into two main activities:

Field activities and laboratory activities. I- Field activities: Field surveys were carried out in which II mosquito larval breeding places were described. They are distributed in II geographically different localities which are belonging to 6 Governorates namely; Port-Said, Ismailiya, North Sinai, Qaluopiya, Guiza and El- Faiyoum. Each larval breeding place was fully described and typed. Six main larval breeding types were reported, these are: Seepage, Sewery trunch cesspit, deserted irrigation canal, deserted wells (Sakia), Cesspool and drainage canal. Each larval breeding place was surveyed for the prevalent mosquito species. Results revealed the presence of larvae, which belong to 13 mosquito species, namely; Anopheles pharoensis, Anopheles muiticolor, Anopheles sergentii, Culex pipiens, Culex univittatus, Culex antennatus, Culex theileri, Culex pusillus, Aedes caspius; Aedes detritus, Cu/eceta longiareolata, Culeceta subochrea, and Uranotanea unguiculata. Nine of the 13 recorded mosquito species were found in seepage water. Water samples were taken from each surveyed breeding place for the isolation of the mosquitocidal bacteria and the associated spore-forming ones. Other water samples were also taken and transported to the laboratory for physical and chemical analysis.