IMMUNOHISTOCHEMICAL EXPRESSION OF PODOPLANIN AND P63 IN ORAL SQUAMOUS CELL CARCINOMA
BAHAA SAMI KARIM;
Abstract
Oral squamous cell carcinoma is the most common intraoral cancer. Oral cancer development is a multistep process with accretion of genetic, epigenetic and metabolic alterations resulting from exposure to carcinogens. Metastatic spread to regional lymph nodes through the lymphatic system is considered one of the major pathways by which SCC disseminates [3].
This study was conducted in order to detect the immunohistochemical expression of a tumor suppressor gene, p63 and a lymphatic marker podoplanin in a group of oral squamous cell carcinomas. Podoplanin and P63 markers were selected because they might have a diagnostic as well as a prognostic value in OSCC [28, 97].
The material of this study consisted of twenty-five formalin-fixed, paraffin-embedded specimens of oral squamous cell carcinoma. Ten cases were diagnosed well differentiated OSCC, nine diagnosed as moderately differentiated OSCC and six diagnosed as poorly differentiated OSCC.
A streptavidin-biotin immunoperoxidase staining system was used for immuno-detection where each lesion was stained with antibody against podoplanin and p63. For all cases, the area percent of immunopositivity for four different microscopic fields was measured. The mean area percent was then calculated and used for statistical analysis.
Immunohistochemical results for this study revealed that the mean area percent of p63 immunopositivity was higher in MDSCC than WDSCC while PDSCC showed the lowest value. ANOVA test revealed a statistically significant difference in mean area percent of podoplanin immunopositivity in different grades of OSCC.
Post hoc multiple comparison test revealed a statistically significant increase of mean area percent of podoplanin immunopositivity in WDSCC and MDSCC when compared to that of PDSCC. On the other hand, a statistically insignificant difference was noted when comparing the mean area percent of podoplanin immunopositivity of WDSCC to that of MDSCC.
The mean area % of p63 immunopositivity was higher in PDSCC than MDSCC while WDSCC showed the lowest value. ANOVA test revealed a statistically significant difference in mean area percent of p63 immunopositivity in different grades of OSCC.
Post hoc multiple comparison test revealed a statistically significant increase of mean area percent of p63 immunopositivity in WDSCC when compared to that of PDSCC. On the other hand, a statistically insignificant difference was noted when comparing the mean area% of p63 immunopositivity of WDSCC and PDSCC to that of MDSCC.
Pearson’s correlation (r) revealed that in WDSCC and MDSCC there was no correlation between the expression of podoplanin and p63, while a weak positive correlation existed when correlating the expression of podoplanin to that of p63 in PDSCC.
This study was conducted in order to detect the immunohistochemical expression of a tumor suppressor gene, p63 and a lymphatic marker podoplanin in a group of oral squamous cell carcinomas. Podoplanin and P63 markers were selected because they might have a diagnostic as well as a prognostic value in OSCC [28, 97].
The material of this study consisted of twenty-five formalin-fixed, paraffin-embedded specimens of oral squamous cell carcinoma. Ten cases were diagnosed well differentiated OSCC, nine diagnosed as moderately differentiated OSCC and six diagnosed as poorly differentiated OSCC.
A streptavidin-biotin immunoperoxidase staining system was used for immuno-detection where each lesion was stained with antibody against podoplanin and p63. For all cases, the area percent of immunopositivity for four different microscopic fields was measured. The mean area percent was then calculated and used for statistical analysis.
Immunohistochemical results for this study revealed that the mean area percent of p63 immunopositivity was higher in MDSCC than WDSCC while PDSCC showed the lowest value. ANOVA test revealed a statistically significant difference in mean area percent of podoplanin immunopositivity in different grades of OSCC.
Post hoc multiple comparison test revealed a statistically significant increase of mean area percent of podoplanin immunopositivity in WDSCC and MDSCC when compared to that of PDSCC. On the other hand, a statistically insignificant difference was noted when comparing the mean area percent of podoplanin immunopositivity of WDSCC to that of MDSCC.
The mean area % of p63 immunopositivity was higher in PDSCC than MDSCC while WDSCC showed the lowest value. ANOVA test revealed a statistically significant difference in mean area percent of p63 immunopositivity in different grades of OSCC.
Post hoc multiple comparison test revealed a statistically significant increase of mean area percent of p63 immunopositivity in WDSCC when compared to that of PDSCC. On the other hand, a statistically insignificant difference was noted when comparing the mean area% of p63 immunopositivity of WDSCC and PDSCC to that of MDSCC.
Pearson’s correlation (r) revealed that in WDSCC and MDSCC there was no correlation between the expression of podoplanin and p63, while a weak positive correlation existed when correlating the expression of podoplanin to that of p63 in PDSCC.
Other data
| Title | IMMUNOHISTOCHEMICAL EXPRESSION OF PODOPLANIN AND P63 IN ORAL SQUAMOUS CELL CARCINOMA | Other Titles | دراسة مناعية هيستوكيميائية لتحديد بروتينات بودوبلانين وب 63 في سرطان الخلايا الحرشفية الفموي | Authors | BAHAA SAMI KARIM | Issue Date | 2016 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.