Effect Of Roxithromycin Versus Nimesulide OnCyclosporin A- Induced Gingival Overgrowth-Experimental Study
Basma Abdelrahman Ahmed;
Abstract
Cyclosporin A is used, not only in transplantation practice, but also in autoimmune disorders causing various side effects in the oral cavity including GO.
Roxithromycinis a macrolide antibiotic which was proved to have an anti- inflammatory effect beside its antibacterial action. In addition, Roxithromycin was previously used in treatment of cases of periodontitis and CsA-GO.
Nimesulide is a NSAID that acts through selective inhibition of COX2. The anti-inflammatory and analgesic effects of Nimesulide and other COX2 inhibitors has been widely shown in cases such as rheumatoid arthritis, inflammatory bowel diseases, headache, toothache, upper respiratory tract infections and periodontitis.
This study was conducted to evaluate and compare the effect of Roxithromycin and Nimesulide on CsA – GO in albino rats.
Forty male albino rats, weighing 120 to 150 grams were used in the experiment. The animals were randomly divided into 4 groups, ten rats in each group:
Group 1 (Control):
Ten rats were not exposed to any kind of treatment for six weeks.
Group 2 (CsA):
Ten rats injected subcutaneously in the back with 10 mg/ kg CsA once daily, for six weeks.
Group 3 (CsA+ Roxithromycin):
Ten rats injected subcutaneously with 10 mg/kg CsA once daily for six weeks and from the beginning till the end of the 6th week were treated with 40 mg /kg roxithromycin once daily by gastric feeding.
Group 4 (CsA + Nimesulide):
Ten rats injected subcutaneously with 10 mg/kg CsA once daily for six weeks and from the beginning till the end of the 6th week were treated with 5.5 mg /kg Nimesulide once daily by gastric feeding.
At the end of the 6th week, the rats were sacrificed separately by overdose of anesthesia (ketamine) and their heads were immediately dissected to obtain the molar area of both sides of the mandible.
The specimens were fixed immediately in 10% buffered formalin solution, decalcified and embedded in paraffin wax blocks. Bucco-lingual sections were obtained and stained with H and E for histomorphometric analysis comprising measurement of epithelial thickness (ET), connective tissue (CT) height and width, density of fibrous tissue (FTD), fibroblast like-cell and inflammatory cell countsin addition to the amount of bone resorption.
Other buccolingual sections were obtained and stained by anti-laminin antibody for investigation of the number of breaks along the BM in different groups.
The results of Tukey andMann-Whitney tests were as follows:
A statistically significant reduction in the ET (p value < 0.001), FTD (p value < 0.001), CT height and width (p value= 0.001,=0.006, respectively), number of inflammatory and fibroblast- like cells (p value< 0.001 and < 0.001, respectively), alveolar bone loss (p value= 0.002) and the number of gingival BM breaks (p value= 0.004) was observed when G3 was compared to G2.
In addition, G4 revealed a statistically significant reduction in the ET, FTD and CT height (p value < 0.001,< 0.001, and < 0.001 respectively), while the CT width decreased insignificantly. Also, the number of fibroblast- like cells, inflammatory cells, amount of bone resorption and number of BM breaks decreased with statistical significant relations when compared to G2 (p value= 0.004,< 0.001, =0 .009 and < 0.001 respectively).
Furthermore, the results showed no statistically significant difference between Roxithromycin and Nimesulide in treatment of CsA-GO at both the histomorphometric and immunohistochemical levels where the p values regarding ET, FTD, CT height, CT width, numbers of fibroblast- like and inflammatory cells, amount of bone resorption and number of gingival BM breaks were as follows (p value= 0.994, 0.594, 0.842, 0.407, 0.645, 0.403, 0.934 and 0.481 respectively).
Roxithromycinis a macrolide antibiotic which was proved to have an anti- inflammatory effect beside its antibacterial action. In addition, Roxithromycin was previously used in treatment of cases of periodontitis and CsA-GO.
Nimesulide is a NSAID that acts through selective inhibition of COX2. The anti-inflammatory and analgesic effects of Nimesulide and other COX2 inhibitors has been widely shown in cases such as rheumatoid arthritis, inflammatory bowel diseases, headache, toothache, upper respiratory tract infections and periodontitis.
This study was conducted to evaluate and compare the effect of Roxithromycin and Nimesulide on CsA – GO in albino rats.
Forty male albino rats, weighing 120 to 150 grams were used in the experiment. The animals were randomly divided into 4 groups, ten rats in each group:
Group 1 (Control):
Ten rats were not exposed to any kind of treatment for six weeks.
Group 2 (CsA):
Ten rats injected subcutaneously in the back with 10 mg/ kg CsA once daily, for six weeks.
Group 3 (CsA+ Roxithromycin):
Ten rats injected subcutaneously with 10 mg/kg CsA once daily for six weeks and from the beginning till the end of the 6th week were treated with 40 mg /kg roxithromycin once daily by gastric feeding.
Group 4 (CsA + Nimesulide):
Ten rats injected subcutaneously with 10 mg/kg CsA once daily for six weeks and from the beginning till the end of the 6th week were treated with 5.5 mg /kg Nimesulide once daily by gastric feeding.
At the end of the 6th week, the rats were sacrificed separately by overdose of anesthesia (ketamine) and their heads were immediately dissected to obtain the molar area of both sides of the mandible.
The specimens were fixed immediately in 10% buffered formalin solution, decalcified and embedded in paraffin wax blocks. Bucco-lingual sections were obtained and stained with H and E for histomorphometric analysis comprising measurement of epithelial thickness (ET), connective tissue (CT) height and width, density of fibrous tissue (FTD), fibroblast like-cell and inflammatory cell countsin addition to the amount of bone resorption.
Other buccolingual sections were obtained and stained by anti-laminin antibody for investigation of the number of breaks along the BM in different groups.
The results of Tukey andMann-Whitney tests were as follows:
A statistically significant reduction in the ET (p value < 0.001), FTD (p value < 0.001), CT height and width (p value= 0.001,=0.006, respectively), number of inflammatory and fibroblast- like cells (p value< 0.001 and < 0.001, respectively), alveolar bone loss (p value= 0.002) and the number of gingival BM breaks (p value= 0.004) was observed when G3 was compared to G2.
In addition, G4 revealed a statistically significant reduction in the ET, FTD and CT height (p value < 0.001,< 0.001, and < 0.001 respectively), while the CT width decreased insignificantly. Also, the number of fibroblast- like cells, inflammatory cells, amount of bone resorption and number of BM breaks decreased with statistical significant relations when compared to G2 (p value= 0.004,< 0.001, =0 .009 and < 0.001 respectively).
Furthermore, the results showed no statistically significant difference between Roxithromycin and Nimesulide in treatment of CsA-GO at both the histomorphometric and immunohistochemical levels where the p values regarding ET, FTD, CT height, CT width, numbers of fibroblast- like and inflammatory cells, amount of bone resorption and number of gingival BM breaks were as follows (p value= 0.994, 0.594, 0.842, 0.407, 0.645, 0.403, 0.934 and 0.481 respectively).
Other data
| Title | Effect Of Roxithromycin Versus Nimesulide OnCyclosporin A- Induced Gingival Overgrowth-Experimental Study | Other Titles | تأثير الروكزيثروميسين مقابل النيموسوليدعلى تضخم اللثة الناتج عن إستخدام سيكلوسبورين أ :دراسةتجريبية | Authors | Basma Abdelrahman Ahmed | Issue Date | 2015 |
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