(Studies on Coccidian parasites (Cryptosporidiun1

Abstract

The separation of Cryptosporidium parvum with Cesium chloride collected the oocysts in one sharp zone Cryptosporidium antigens showed reaction using isoelectric focusing at PI 4.1 for English and Egyptian calves and two z:c:meof-ieacticiDS at PI 4.8 - and 5 for Egyptian goats. •

Using of SDS-PAGE showed 15 similar molecular weight . - bands at the range between 110 Kda and- 15 Kda for- C. Parvum

(English and Egyptian calves. While C.Parvum from Egyptian goats showed only 5 bands at 110, 70, 54, 15 and 13 Kda. Western blotting was applied using homogenous 20% Gel C.Parvum (English calves strain) reacted with the posiutive sera in 5 bands (MWs, 120, 81, 54, 43 and 11 Kda). While the Egyptian calves reacted at 11 bands at MWs ranged from (160 and 11 Kda). C. Parvum (Egyptian goats strain) showed 6 clear bands at 94, 81, 70, 54, 14 and 11 Kda.