Potentiality of Hard Tissue Formation After Application of Stem Cells in Furcation Perforation (An Animal study)
Hala Fayek Shaker Mohammed;
Abstract
The potentiality of hard tissue formation in furcation perforation using stem cells in animals was evaluated in this study .This study utilized six male adult healthy mongrel dogs. The mandibular first molar teeth were bilaterally extracted in each dog as a source for dental pulp stem cells and transported to the laboratory for stem cell harvesting and culturing.
Dental pulp was and removed washed then minced into 2x2x1 mm fragments using a sterile scalpel, then digested by collagenase enzyme type I to acquire a cells that were cultured and incubated at 37C in 5 % CO2 and monitored every day for need of change of culture medium. Day 8 cells were collected.
Collagraft Bone Graft Matrix Strip sterile in 2mm length x2 mm width x2 mm height impregnated with DPSCS of cell density 5x106 and DMP1was used to repair a freshly induced furcation perforation after endodontic root canal treatment in the premolars P2, P3 and P4 premoalrs and second molar teeth in each of the two quadrants. Right Mandibular second premolar P2 was used as a negative control group and Left Mandibular second premolar P2 was used as a positive control group in each dog. Teeth were restored and the animals were followed up for observation periods one month (2 dogs), two months (2 dogs) and three months (2 dogs).
At the end of every observation period the dogs were sacrificed and the mandibles were surgically dissected, cut and prepared for sectioning, staining and diagnosis. Sections were stained with hematoxylin and eosin (H&E) Masson's trichrome stain and Alizarin Red and Parameters were evaluated through the three observation periods
Dental pulp was and removed washed then minced into 2x2x1 mm fragments using a sterile scalpel, then digested by collagenase enzyme type I to acquire a cells that were cultured and incubated at 37C in 5 % CO2 and monitored every day for need of change of culture medium. Day 8 cells were collected.
Collagraft Bone Graft Matrix Strip sterile in 2mm length x2 mm width x2 mm height impregnated with DPSCS of cell density 5x106 and DMP1was used to repair a freshly induced furcation perforation after endodontic root canal treatment in the premolars P2, P3 and P4 premoalrs and second molar teeth in each of the two quadrants. Right Mandibular second premolar P2 was used as a negative control group and Left Mandibular second premolar P2 was used as a positive control group in each dog. Teeth were restored and the animals were followed up for observation periods one month (2 dogs), two months (2 dogs) and three months (2 dogs).
At the end of every observation period the dogs were sacrificed and the mandibles were surgically dissected, cut and prepared for sectioning, staining and diagnosis. Sections were stained with hematoxylin and eosin (H&E) Masson's trichrome stain and Alizarin Red and Parameters were evaluated through the three observation periods
Other data
| Title | Potentiality of Hard Tissue Formation After Application of Stem Cells in Furcation Perforation (An Animal study) | Other Titles | احتمالية تكوين أنسجة صلبة بعد وضع الخلاية الجذعية فى ثقب بمنطقة انقسام الجذر (دراسة حيوانية) | Authors | Hala Fayek Shaker Mohammed | Issue Date | 2017 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.