Physiological and Histological Studies on the Effect of Echinacea purpurea in Gamma Irradiated Male Rats
Nabila Safwat Riad;
Abstract
Echinacea purpurea (EP) is a purple cone flower containing phenols, caffeic acid derivatives, alkamides, flavonoids, and essential oils. The present study was designed to investigate the prophylactic and therapeutic effects of EP on gamma radiation-induced oxidative stress in liver and testis tissues and some serum metabolic disturbances in parallel to histopathological changes in liver and testis tissues.
Rats received EP (50 mg/day/Kg body weight) by gavages for 28 successive days either before or after whole body exposure to 3Gy gamma–radiation (one shot dose). A total of 50 rats were divided into 5 groups.
1- Control group: Rats received 1ml distilled water by gavages for 28 consecutive days.
2- EP-treated group: Rats received EP (50 mg/kg body weight/day) for 28 consecutive days and sacrificed 3 days post the last dose of EP.
3- Irradiated group: Rats were whole body exposed to 3 Gy gamma irradiation applied as one shot dose and sacrificed 3 days post irradiation .
4- EP + irradiated group: Rats received EP during 28 consecutive days by gavages before whole body exposure to gamma irradiation at 3 Gy and sacrificed 3 days post irradiation .
5- Irradiated + EP group: Rats received EP during 28 consecutive days by gavages after whole body exposure to gamma irradiation at 3 Gy and sacrificed 3days post the last dose of EP.
For biochemical analysis, blood was collected and sera obtained for testing ALT and AST activities, Glucose, Testosterone, Triglycerides, Total cholesterol, LDL-C, and HDL-C.
Oxidative stress was assessed in liver and testis tissues by measurement of TBARS content, SOD and CAT activities and GSH content.
For histological examination 6 µm thick serial paraffin sections of the liver and testis of rats from each group were prepared, stained with heamatoxylin and eosin and examined by Olympus light microscope.
The results obtained in the current study revealed that administration of EP to normal healthy rats has no effect on TBARS level, SOD and CAT activities as well as GSH content in the liver and testis compared to control.
Rats received EP (50 mg/day/Kg body weight) by gavages for 28 successive days either before or after whole body exposure to 3Gy gamma–radiation (one shot dose). A total of 50 rats were divided into 5 groups.
1- Control group: Rats received 1ml distilled water by gavages for 28 consecutive days.
2- EP-treated group: Rats received EP (50 mg/kg body weight/day) for 28 consecutive days and sacrificed 3 days post the last dose of EP.
3- Irradiated group: Rats were whole body exposed to 3 Gy gamma irradiation applied as one shot dose and sacrificed 3 days post irradiation .
4- EP + irradiated group: Rats received EP during 28 consecutive days by gavages before whole body exposure to gamma irradiation at 3 Gy and sacrificed 3 days post irradiation .
5- Irradiated + EP group: Rats received EP during 28 consecutive days by gavages after whole body exposure to gamma irradiation at 3 Gy and sacrificed 3days post the last dose of EP.
For biochemical analysis, blood was collected and sera obtained for testing ALT and AST activities, Glucose, Testosterone, Triglycerides, Total cholesterol, LDL-C, and HDL-C.
Oxidative stress was assessed in liver and testis tissues by measurement of TBARS content, SOD and CAT activities and GSH content.
For histological examination 6 µm thick serial paraffin sections of the liver and testis of rats from each group were prepared, stained with heamatoxylin and eosin and examined by Olympus light microscope.
The results obtained in the current study revealed that administration of EP to normal healthy rats has no effect on TBARS level, SOD and CAT activities as well as GSH content in the liver and testis compared to control.
Other data
| Title | Physiological and Histological Studies on the Effect of Echinacea purpurea in Gamma Irradiated Male Rats | Other Titles | دراسات فسيولوجية و هستولوجية على تأثير الاكينسيا باربيورا فى ذكور الجرذان المشععة | Authors | Nabila Safwat Riad | Issue Date | 2014 |
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