CYTOGENETIC STUDY OF UTERINE LEIOMYOMATA

Abstract

This work has been done to find out any correlation betweeh clinical characteristics of uterine leiomyomas and the cytogenetic rearrangements, to determine the cytogenetically defined critical regions of chromosomes,hoping to demonstrate the presence I

a possible candidate gene at a specific locus involved in t e development of leiomyomas. This study included randomly selected 25 uterine leiomyoma specimens obtained from 16 patients during hystrectomy dr

myomectomy operations at Obstetrics and Gynaecology Department !

Tanta University Hospital and had not received any

therapy in the last six months.

horomonal I '

Before admission to this study, every woman was subjected Jo

complete history taking, general and local examination, meticulus I ultrasonic evaluation, routine laboratory investigations and pre-

operative preparation. I

The main presenting symptoms and their durations we e recorded. At time of surgery, the number and location of myomas weL

recorded and their maximum cross

section diameter wJs I

measured. Fresh tissue tumour specimens were obtained I aseptically and collected in sterile transport medium then transported immediatly to the cytogenetic laboratory where the were processed to setup tissue cultures according to thb I technique of (Rein et al., 1991)067) and (Ram et al., 1995)(168). The cytogenetic analysis was performed according to ISCN(l995)(32).