Bacterial reduction after root canal preparation using single and multifile preparation systems with different activation methods (An in vitro study)

Abstract

The present study aimed to compare the efficiency of single file system (Neoniti) versus multifile system (Protaper Universal) in reducing Enterococcus Faecalis biofilm in the root canals using different methods of activation of irrigation (conventional syringe, passive ultrasonic irrigation and vibringe). Fifty – four human extracted mandibular permanent first and second molars were selected. The mesial root of each molar was separated at the bifurcation. Root canals selected were type III. 48 roots were divided into 2 equal groups (n=24) and they were equally distributed in length. Root canals were enlarged to ISO # 20. Smear layer was removed. They were packed in two separate sterilization pouches and steam autoclaved at 121 °C for 30 minutes. A clinical isolate of E. faecalis from the Microbiology laboratory was used for biofilm formation. Monitoring of bacterial biofilm development onto root canal dentin was assessed by SEM examination after three weeks using the positive control group (n=3).

 3 samples were served as the negative control group to check for the sterility of the procedures and 3 samples were served as the positive control group to check for bacterial viability throughout the experiment & used as indicators for biofilm formation.