Microbial Production of Some Metallic Nanoparticles and Their Application as Antitumor

Abstract

This study aims to use well identified different microorganisms as economic and healthy for biosynthesis of AgNPs to solve problem of toxicity of by-product of toxic chemicals used in chemical method. Five cultures, two fungal cultures (Fusarium oxysporum f. sp. lycopersici (EMCC 632) and Trichoderma viride (EMCC 107)) and three bacterial cultures (Pseudomonas aeruginosa (DSM 50090), Lactobacillus rhamnosus (ATCC 7479) and Lactobacillus helveticus (CCM 7193)), were used. These cultures exhibited high ability for biosynthesis extracellular enzymes which were chosen as the pure cultures for NPs synthesis. 1- The first screening program for AgNPs synthesis by these five cultures as follows: a) Incubate equal weight 10 g from each used fungal culture with solution of silver nitrate with concentration of 1M for 5 days at temperature 28ºC. b) Incubate each screened bacterial culture with solution of silver nitrate with concentration of 1M for 72 h at temperature 28ºC. 2- Study the ability of each culture to biosynthesis AgNPs and selected the most effective culture for AgNPs Summary 94 synthesis with the smallest size through measuring the particle size for each result. 3- Observation of AgNPs intracellularly by Confocal Laser Scanning Microscopy and Transmission Electron Microscopy. 4- Study the factors affecting on controlling of AgNPs size synthesized by F. oxysporum: a) Optimum silver nitrate concentration for F. oxysporum was found to be 10-2 M for smallest AgNPs synthesis. b) The smallest size of AgNPs showed under static condition. c) Optimum incubation temperature was found to be 50º C for smallest AgNPs synthesis. d) The optimum wet weight of F. oxysporum was 11 g. e) The optimal pH value was found to be 6 for smallest