APPLICATION OF SOME NANOTECHNOLOGICAL APPROACHES IN RAPID DETECTION OF FEED-BORNE PATHOGENIC BACTERIA

Abstract

This study was planned to exploit the most recently innovated noble nanoparticles in a highly sensitive colorimetric real-time-assay for detecting E. coli O157:H7 in feedstuffs. Gold nanoparticles (AuNPs) were prepared adopting the citrate method and the obtained colloidal nanoparticle suspension was characterized using the UV–vis spectroscopy, Dynamic light scattering (DLS), Zeta potential instrument, Transmission Electron Microscopy (TEM) and X’Pert PRO X-ray Diffraction System (XRD). Results showed that the prepared AuNPs were well dispersed colloidal spherical shaped nanosized particles averaged 40 nm in size having a characteristic surface plasmon resonance band similar to gold nanoparticles and absorbance peak of 530 nm. Zeta potential of the prepared AuNPs verified the negatively charged surfaces of -32 mv. Specific E. coli O157:H7 antiserum was conjugated to the surfaces of the prepared AuNPs and the developed AuNPs-conjugated suspension had a red color. Stability of the AuNPs