Isolation, Purification and Characterization of a Lipase from Thermophilic Bacteria

Abstract

Fifty one bacterial isolates were isolated from Egyptian soil. These isolates were purified, and were examined for their ability to produce extracellular enzymes such as lipases, amylases, proteases and uricases. Four promising isolates were chosen based on their ability to produce lipolytic enzymes with significant level. These four isolates were abbreviated as 5, Ao, A72 and B72. After several rounds of screening, isolate 5 was selected as the promising lipase producing bacteria. Bacterial isolates were identified using biochemical tests as thermophilic Geobacillus. Further confirmation of biochemical characterization was carried out for isolate 5 utilizing 16S rRNA technique. Data were submitted to the GeneBank sequence database with a given accession number DQ923400. The bacterial isolate was identified as Geobacil/us stearothermophilus