-•Molecular Studies and AntibiOtic sensitivity Of E.coli Isolated From differer1t hosts
Amira. Zal
Abstract
E.coli being a normal gastrointestinal inhabitant, it is also associated with a variety of pathological conditions in animals. (Smith.1963). in calves the infection takes form of scour which could be watery or bloody.
In E.coli, virulence is associated with various chromosomal and plasmid-born genes, which encode heat-labile (LTs) and heat-stable entero toxins (STs), shiga toxins. (Stx1 and stx2), enteroheamolysin, intestinal adherence factors, and various pili.
This work was planned to phenotypic and some of genotypic manner of antibiotic resistance ofE.coli.
I
We carried out the following:
1. Isolation and identification of E.coli from different sources (cattle, sheep, chicken).
2. Determining the hemolytic activity of some E.coli isolates.
3. Studying the susceptibility of the E.coli isolates to antimicrobial agents.
4. Serotyping the isolated E.coli.
5. Application of polymerase chain reaction (PCR) to confirm the presence of the most commonly antibiotic resistant gene of the isolated E.coli.
This study was carried-out on :
A total of(35) rectal swabs (23 from calves and 12 from sheep) were collected. Moreover, (31) coloacal swabs were collected from broilers. The samples were collected from different localities in Behaira governorate. Samples were labeled and transported with minimum of delay in an ice box to the laboratory of Department of Microbiology, Fac. of Vet. Med., Alex. University during the period of November 2008 till April 2009.
This study concluded that :
Out of 35 rectal swabs (23 from calves and 12 from sheep) E.coli were isolated at a percentage of 69.66% and 66.76% respectively. Moreover, 31 E.coli were isolated at percentage ofl9.35% from 31 cloacal swabs from chickens.
A total of 30 identified E.coli isolates were cultivated on blood agar medium, 11 isolates of them 36.66% showed no heamolysis.
The eleven isolates which showed u-heamolysis and -heamolysis phenotype wee from cattle 3 (18.75%) produced u-heamolysis and 1 (6.25%) produced -heamolysis and from sheep 3 (37.5%) produced -heamolytic isolates and from chicken 1 (16.66%) was u-heamolysin and 3 (50%) were -heamolytic isolates.
Results of serological identification of the isolated E.coli from different hosts. Seventeen E.coli isolates that were chosen randomly were identified using monovalent and polyvalent 0- anti-sera.Eight E.coli isolates from cattle were serologically typed and 8 were untyped.
In E.coli, virulence is associated with various chromosomal and plasmid-born genes, which encode heat-labile (LTs) and heat-stable entero toxins (STs), shiga toxins. (Stx1 and stx2), enteroheamolysin, intestinal adherence factors, and various pili.
This work was planned to phenotypic and some of genotypic manner of antibiotic resistance ofE.coli.
I
We carried out the following:
1. Isolation and identification of E.coli from different sources (cattle, sheep, chicken).
2. Determining the hemolytic activity of some E.coli isolates.
3. Studying the susceptibility of the E.coli isolates to antimicrobial agents.
4. Serotyping the isolated E.coli.
5. Application of polymerase chain reaction (PCR) to confirm the presence of the most commonly antibiotic resistant gene of the isolated E.coli.
This study was carried-out on :
A total of(35) rectal swabs (23 from calves and 12 from sheep) were collected. Moreover, (31) coloacal swabs were collected from broilers. The samples were collected from different localities in Behaira governorate. Samples were labeled and transported with minimum of delay in an ice box to the laboratory of Department of Microbiology, Fac. of Vet. Med., Alex. University during the period of November 2008 till April 2009.
This study concluded that :
Out of 35 rectal swabs (23 from calves and 12 from sheep) E.coli were isolated at a percentage of 69.66% and 66.76% respectively. Moreover, 31 E.coli were isolated at percentage ofl9.35% from 31 cloacal swabs from chickens.
A total of 30 identified E.coli isolates were cultivated on blood agar medium, 11 isolates of them 36.66% showed no heamolysis.
The eleven isolates which showed u-heamolysis and -heamolysis phenotype wee from cattle 3 (18.75%) produced u-heamolysis and 1 (6.25%) produced -heamolysis and from sheep 3 (37.5%) produced -heamolytic isolates and from chicken 1 (16.66%) was u-heamolysin and 3 (50%) were -heamolytic isolates.
Results of serological identification of the isolated E.coli from different hosts. Seventeen E.coli isolates that were chosen randomly were identified using monovalent and polyvalent 0- anti-sera.Eight E.coli isolates from cattle were serologically typed and 8 were untyped.
Other data
| Title | -•Molecular Studies and AntibiOtic sensitivity Of E.coli Isolated From differer1t hosts | Other Titles | دراسات جزيئية والحساسية للمضادات الحيوية للميكروب القولونى المعزول من عوائل مختلفة | Authors | Amira. Zal<i Seliem Zayan | Issue Date | 2011 |
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