An Immunohistochemical Study of Laminin in Basal & Squamous Cell Carcinomas

Abstract

Laminins are components of the extracellular matrix that contribute to the architecture of the basal lamina, and mediate cell adhesion, growth, migration, proliferation, and differentiation (Hao et al,2001). Carcinomas are malignancies derived from epithelial cells. In the progression of carcinomas, the role of laminins and BM is not well understood. BM laminins, in particular, may play a role in enhancing carcinoma cell motility (Tani et aL, 1997).

The aim of this study was to evaluate the distribution pattern of laminin in both BCC and SCC and to correlate laminin distributon with different variants of BCC and to correlate laminin distribution with metastatic potential in SCC. This study included 38 patients (21 BCC, 17 SCC). Skin biopsy specimens were obtained from all patients for routine

histopathological and immunohistochemical study. Palpable lymph nodes were also obtained for routine histopathological and inununohistochemical study. Twenty five cases (6 healthy skin + 19 specimens from safety margins) served as control group. Laminin was evaluated qualitatively and semiquantitatively using monoclonal mouse antihuman antibody, in different variants of BCC and SCC.