Direct lmmunofluoresence Staining versus Cell Culture Method In Diagnosis of '" Adenovirus Keratoconjunctivits

Abstract

Human adenoviruses are non-enveloped DNA VIruses. Adenovirus infection occurs worldwide and cases a wide range of clinical diseases that affect ocular, gastrointestinal and respiratory system. Ocular adenoviral infections exhibit varieties of clinical presentations, such as epidemic keratoconjunctivitis and pharyngoconjunctival fever.

Adenovirus infection causes a variety of clinical diseases that affect ocular, gastrointestinal and respiratory systems. Ocular adenoviral infection has two clinical forms epidemic keratoconjuctivitis and pharryngoconjunctival fever.

Laboratory diagnosis of adenovirus infection depends on direct electron microscopic examination, viral isolation, DNA or antigen detection by immunofluorscent staining or detection of serum specific antibodies and detection of DNA by polymerase chain reaction (PCR).

The mm of the present study was to evaluate the incidence of adenovirus keratoconjunctivitis by using immunotluorscent staining of direct swabs and immunotluorscent staining after cell culture and compare between the results. • Adenoviral antigens were detected In 45 cases (90%) out of 50 cases having viral conjunctivitis by using immunotluorscent staining of direct swabs and 30 cases (60%) of them were positive for the presence of adenovirus antigen in their specimens, by using cell culture technique, while adenoviral antigens were detected also in 33 cases (66%) by using immunotluorscent staining after cell culture.