STUDIES ON THE VEGETATIVE PROPAGATION OF PEACH TREES
GHADA MAHMOUD SOLIMAN;
Abstract
The present study was carried out in Strawberry Tissue Culture Laboratory and Non- Traditional Crops, Improvement Center, Faculty of Agriculture, Ain Shams University during
2000 and 2001 years. Hansen 536 peach rootstock explants of
the renewed 7-year old trees grown in the same faculty were the plant material used for this dissertation. Hence, it was hoped to find out an ideal method as true propagation method for providing with the adequate quantities of nursery transplants of this desired rootstock to meet the increasing demand due to its advantages, especially under the new reclaimed area condition so, some treatments dealing with the various stages of direct regeneration through tissue culture technique i.e, establishment; proliferation, rooting and acclimatization were investigated as follows.
V.l.Establishment stage: -
In this regard a factorial experiment was devoted for studying the effect of explant type (shoot tip and stem node cutting) combined with surface sterilization of these explants by soaking in diluted solution (10 or 20%) of sodium hypochlorite (commercial bleach "Clorox") for either 10, 15, or 20 min. were investigated regarding their influence on contamination; browning and survival% during this stage.
Data were recorded after 4 weeks from culturing on MS base medium supplemented with 3% sucrose and 0.7% agar with adjusted pH at 5.6- 5.8.
V.2.Proliferation "shoot multiplication" stage:-
In this stage four weeks old aseptically growing explants obtained from the establishment stage were used as source for the multiplication stage.
2000 and 2001 years. Hansen 536 peach rootstock explants of
the renewed 7-year old trees grown in the same faculty were the plant material used for this dissertation. Hence, it was hoped to find out an ideal method as true propagation method for providing with the adequate quantities of nursery transplants of this desired rootstock to meet the increasing demand due to its advantages, especially under the new reclaimed area condition so, some treatments dealing with the various stages of direct regeneration through tissue culture technique i.e, establishment; proliferation, rooting and acclimatization were investigated as follows.
V.l.Establishment stage: -
In this regard a factorial experiment was devoted for studying the effect of explant type (shoot tip and stem node cutting) combined with surface sterilization of these explants by soaking in diluted solution (10 or 20%) of sodium hypochlorite (commercial bleach "Clorox") for either 10, 15, or 20 min. were investigated regarding their influence on contamination; browning and survival% during this stage.
Data were recorded after 4 weeks from culturing on MS base medium supplemented with 3% sucrose and 0.7% agar with adjusted pH at 5.6- 5.8.
V.2.Proliferation "shoot multiplication" stage:-
In this stage four weeks old aseptically growing explants obtained from the establishment stage were used as source for the multiplication stage.
Other data
| Title | STUDIES ON THE VEGETATIVE PROPAGATION OF PEACH TREES | Other Titles | دراسات على الاكثار الخضرى لاشجار الخوخ | Authors | GHADA MAHMOUD SOLIMAN | Issue Date | 2004 |
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