Molecular biological studies on Escherichia coli isolated from diarrheic and apparently healthy calves
Noha Mohamed Bakry Nady;
Abstract
This study was carried out in different farms located in Giza and Gharbiya governorates and farms on Cairo-Alexandria Desert Road. The aim of this work is to study neonatal calf diarrhea caused by pathogenic E. coli in diarrheic calves.
The study was performed to achieve the following:
1. Detection of E. coli causing diarrhea in calves through bacteriological examination of fecal samples collected from diarrheic and apparently healthy in-contact cattle and buffalo calves.
2. Confirmation of isolated E. coli through molecular identification using Polymerase chain reaction.
3. Characterization of genes encoding virulence factors of pathogenic E. coli strains using multiplex PCR and identification of E. coli pathotypes causing diarrhea in calves.
4. Study the effect of age, sex and season on occurrence of pathogenic E. coli in diarrheic and apparently healthy in-contact cattle and buffalo calves.
5. Characterization of genes encoding different serogroups through serogrouping of isolated E. coli using agglutination test and Polymerase chain reaction.
6. Characterization of pathogenic effect of E. coli throug
The study was performed to achieve the following:
1. Detection of E. coli causing diarrhea in calves through bacteriological examination of fecal samples collected from diarrheic and apparently healthy in-contact cattle and buffalo calves.
2. Confirmation of isolated E. coli through molecular identification using Polymerase chain reaction.
3. Characterization of genes encoding virulence factors of pathogenic E. coli strains using multiplex PCR and identification of E. coli pathotypes causing diarrhea in calves.
4. Study the effect of age, sex and season on occurrence of pathogenic E. coli in diarrheic and apparently healthy in-contact cattle and buffalo calves.
5. Characterization of genes encoding different serogroups through serogrouping of isolated E. coli using agglutination test and Polymerase chain reaction.
6. Characterization of pathogenic effect of E. coli throug
Other data
| Title | Molecular biological studies on Escherichia coli isolated from diarrheic and apparently healthy calves | Authors | Noha Mohamed Bakry Nady | Issue Date | 2016 |
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