Real-time PCR in Detection of Mutations Associated with Resistance to Second-Line Drugs in Clinical Isolates of Mycobacterium Tuberculosis
Fatma El Zahraa Youssef fathi;
Abstract
ABSTRACT
Background: According to World Health Statistics, Total cases notified of
TB in Egypt during 2015 was 8,155 new and relapse cases with an
incidence rate of 15 per 100,000 population. Fortunately, Egypt is still not
on the WHO’s list of 30 high-TB-burden countries or the 27 high MDRTB
burden
countries.
In
Egypt,
percentage
of
MDR
among
new
TB
cases
was
14% while that among previously treated TB cases was 23%
according to most recent year available data .Several methods have been
developed for the rapid detection of drug resistance tuberculosis compared
with conventional time consuming drug susceptibility testing (DST).
These methods include genotypic assays which are easy to perform and
shorten the turnout time for the diagnosis of MDR-TB.
Objective: The aim of this study was to evaluate real-time PCR as a rapid
diagnostic method in detection of mutations associated with the resistance
to second-line injectable agents (capreomycin, amikacin and kanamycin)
and fluroquinolones in M. tuberculosis clinical isolates in comparison to
proportional method.
Background: According to World Health Statistics, Total cases notified of
TB in Egypt during 2015 was 8,155 new and relapse cases with an
incidence rate of 15 per 100,000 population. Fortunately, Egypt is still not
on the WHO’s list of 30 high-TB-burden countries or the 27 high MDRTB
burden
countries.
In
Egypt,
percentage
of
MDR
among
new
TB
cases
was
14% while that among previously treated TB cases was 23%
according to most recent year available data .Several methods have been
developed for the rapid detection of drug resistance tuberculosis compared
with conventional time consuming drug susceptibility testing (DST).
These methods include genotypic assays which are easy to perform and
shorten the turnout time for the diagnosis of MDR-TB.
Objective: The aim of this study was to evaluate real-time PCR as a rapid
diagnostic method in detection of mutations associated with the resistance
to second-line injectable agents (capreomycin, amikacin and kanamycin)
and fluroquinolones in M. tuberculosis clinical isolates in comparison to
proportional method.
Other data
| Title | Real-time PCR in Detection of Mutations Associated with Resistance to Second-Line Drugs in Clinical Isolates of Mycobacterium Tuberculosis | Authors | Fatma El Zahraa Youssef fathi | Issue Date | 2017 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.