NEW TECHNIQUES OF PCR
Ehab Mahfouz Ryiad Bottros;
Abstract
The polymerase chain reaction (PCR) first described in
1985, is the most widely used method of nucleic acid amplification and has acquired a pivotal role in medical sciences and clinical laboratory.
In recent years several approaches have been proposed to overcome the draw backs of the standard technique e.g. low specificity and sensitivity, danger of amplifmg contaminant or carry-over DNA sequences and the short size and limiting amounts of product by developing new techniques e.g. long range PCR, anchored PCR, RT-PCR and real time PCR.
PCR is useful in diagnosis of very few and inaccessible pathogen as T.B. also when pathogen can not be cultured as M. leprae. Also PCR is non invasive technique for diagnosis of Helicobactr pylori.
Clinical application of PCR can be used in monitoring many viral infections as HBV, HCV, HIV and CMV.
PCR can be used in detecting minimal residual disease m different haematological malignancies as ALL, CML and Non Hodgkin's lymphoma.
PCR can be used in monitoring MRD in cancer breast. Also in assessment of c-myc mRNA in colorectal cancer.
1985, is the most widely used method of nucleic acid amplification and has acquired a pivotal role in medical sciences and clinical laboratory.
In recent years several approaches have been proposed to overcome the draw backs of the standard technique e.g. low specificity and sensitivity, danger of amplifmg contaminant or carry-over DNA sequences and the short size and limiting amounts of product by developing new techniques e.g. long range PCR, anchored PCR, RT-PCR and real time PCR.
PCR is useful in diagnosis of very few and inaccessible pathogen as T.B. also when pathogen can not be cultured as M. leprae. Also PCR is non invasive technique for diagnosis of Helicobactr pylori.
Clinical application of PCR can be used in monitoring many viral infections as HBV, HCV, HIV and CMV.
PCR can be used in detecting minimal residual disease m different haematological malignancies as ALL, CML and Non Hodgkin's lymphoma.
PCR can be used in monitoring MRD in cancer breast. Also in assessment of c-myc mRNA in colorectal cancer.
Other data
| Title | NEW TECHNIQUES OF PCR | Other Titles | التقنيات الحديثة فى اختبار بى .سى . أر | Authors | Ehab Mahfouz Ryiad Bottros | Issue Date | 2001 |
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