Evaluation of P-element .\Iediated Transformation Vectors for The Expression of Foreign Genes in Eukaryotes
Sayed Mohamed Sayed;
Abstract
This study aims at finding a gene transfer vector that can transfer foreign genes into plant cells and animal cells and studying its ability to express these genes into living organisms. This study was carried out using the transposable ?-element, isolated from Drosophila, and particle bombardment technique (Biolistic gun).
?-element is a transposable element found in the genome of some animals. ?-element can transfer itself from a place to another on the genome so, this phenomenon was utilized in gene transfer to some living organisms. The construct pJKP2 which contains the neomycin resistance gene and the P-galactosidase gene was used as a member of the ?-element transformation vectors family.
Three different plants; faba bean ( Vicia faba L.), squash ( Cucurbita pepo L.) and pepper (Capsicum annuum L.); of economic importance in Egypt were used to assess the ability of
?-element to express genes into these plants. Particle bombardment was carried out using the Biolistic® PDS/1OOOHe device and tungsten as a microcarrier. Bombardment was carried out under
helium pressure of II 00 psi and vacuum of 20 inches of Hg. P
galactosidase activity was detected in the three plants 4 days after bombardment. PCR analysis for the genomic DNA of the bombarded plants gave the expected amplification product (775bp)
?-element is a transposable element found in the genome of some animals. ?-element can transfer itself from a place to another on the genome so, this phenomenon was utilized in gene transfer to some living organisms. The construct pJKP2 which contains the neomycin resistance gene and the P-galactosidase gene was used as a member of the ?-element transformation vectors family.
Three different plants; faba bean ( Vicia faba L.), squash ( Cucurbita pepo L.) and pepper (Capsicum annuum L.); of economic importance in Egypt were used to assess the ability of
?-element to express genes into these plants. Particle bombardment was carried out using the Biolistic® PDS/1OOOHe device and tungsten as a microcarrier. Bombardment was carried out under
helium pressure of II 00 psi and vacuum of 20 inches of Hg. P
galactosidase activity was detected in the three plants 4 days after bombardment. PCR analysis for the genomic DNA of the bombarded plants gave the expected amplification product (775bp)
Other data
| Title | Evaluation of P-element .\Iediated Transformation Vectors for The Expression of Foreign Genes in Eukaryotes | Other Titles | تقييم قدرة بعض النواقل الجينية المحتوية على عنصر -ب على التمثيل الوراثى لبعض الجينات الغربية فى حقيقيات النواه | Authors | Sayed Mohamed Sayed | Issue Date | 1998 |
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