Evaluation of the antimicrobial effect of Miswak in endodontic infections
Nesma Ahmed Hamdi;
Abstract
The first part of this study was to evaluate the antimicrobial effect of Miswak on Enterococcus faecalis biofilm.
Fifty single rooted teeth were used for this study.
The samples were divided into five groups (n=10),all samples were cleaned,sterilized and decoronated to a standard length 18mm.
Four groups were incubated 21 days in a clinical isolate of Enterococcus faecalis to develop a mature bacterial biofilm, only one group was left sterile (negative control).
After 21 days at 37oC each group received its intracanal medicament & marked by a certain color as follows:
Group I was medicated by Miswak & marked with red nail polish, group II was medicated by Triple antibiotic paste & marked with blue, group III was medicated by Ca(OH)2& marked with green and group IV was left without medication& marked with green(positive control).
Then all groups were sealed and maintained in a humid environment at 37oC for one week.
After one week all the samples were irrigated with sterile saline to remove the root canal contents.
Bacterial samples from each sample were taken and cultured to determine the bacterial counting for each sample, then collected data were statistically analyzed.
The results of bacterial count showed that the difference between the tested groups was statistically insignificant.
The second part of the study was to do elemental analysis of Miswak aqueous extract and freshly ground Miswak
powder using ScanningElectron Microscope /Energy Dispersive Spectroscopy(SEM/EDS).
And the results showed that the miswak extract contains many elements that are parts of compounds that could be either antimicrobial by its own effect as Sulfur, Molybdenum and Thallium (85)or form compounds that have an antibacterial effect as Sodium with chlorine or Fluoride, or even all of them have a Synergistic effect that acted on the bacterial biofilm and brought its antimicrobial effect. Also by comparing the results of SEM/EDS analysis of Miswak extract and fresh ground Miswak powder revealed that the concentrations of these elements are slightly higher in the extract.
*Within the limitations of this study, it can be concluded that:
1- Miswak extract can be considered a reliable intracanal medicament.
2- The antimicrobial efficacy of Miswak against E.faecalis is comparable to that of triple antibiotic paste and better than that of calciun hydroxide.
3- The local application of antibacterial agents can be beneficial in resistant cases of apical periodontitis but only after careful culture and sensitivity testing to choose the appropriate agent for the existing flora.
Fifty single rooted teeth were used for this study.
The samples were divided into five groups (n=10),all samples were cleaned,sterilized and decoronated to a standard length 18mm.
Four groups were incubated 21 days in a clinical isolate of Enterococcus faecalis to develop a mature bacterial biofilm, only one group was left sterile (negative control).
After 21 days at 37oC each group received its intracanal medicament & marked by a certain color as follows:
Group I was medicated by Miswak & marked with red nail polish, group II was medicated by Triple antibiotic paste & marked with blue, group III was medicated by Ca(OH)2& marked with green and group IV was left without medication& marked with green(positive control).
Then all groups were sealed and maintained in a humid environment at 37oC for one week.
After one week all the samples were irrigated with sterile saline to remove the root canal contents.
Bacterial samples from each sample were taken and cultured to determine the bacterial counting for each sample, then collected data were statistically analyzed.
The results of bacterial count showed that the difference between the tested groups was statistically insignificant.
The second part of the study was to do elemental analysis of Miswak aqueous extract and freshly ground Miswak
powder using ScanningElectron Microscope /Energy Dispersive Spectroscopy(SEM/EDS).
And the results showed that the miswak extract contains many elements that are parts of compounds that could be either antimicrobial by its own effect as Sulfur, Molybdenum and Thallium (85)or form compounds that have an antibacterial effect as Sodium with chlorine or Fluoride, or even all of them have a Synergistic effect that acted on the bacterial biofilm and brought its antimicrobial effect. Also by comparing the results of SEM/EDS analysis of Miswak extract and fresh ground Miswak powder revealed that the concentrations of these elements are slightly higher in the extract.
*Within the limitations of this study, it can be concluded that:
1- Miswak extract can be considered a reliable intracanal medicament.
2- The antimicrobial efficacy of Miswak against E.faecalis is comparable to that of triple antibiotic paste and better than that of calciun hydroxide.
3- The local application of antibacterial agents can be beneficial in resistant cases of apical periodontitis but only after careful culture and sensitivity testing to choose the appropriate agent for the existing flora.
Other data
Title | Evaluation of the antimicrobial effect of Miswak in endodontic infections | Other Titles | تقييم لتأثيرالمسواك كعقار مضاد للبكتيريا فى القنوات العصبية | Authors | Nesma Ahmed Hamdi | Issue Date | 2015 |
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