ENZYME-LINKED IMMUNO-SORBENT ASSAY VERSUS DIRECT MICROSCOPY IN DIAGNOSIS AND CURE ASSESSMENT OF GIARDIA LAMBLIA INFECTION
Sabah Abd Elgany Ahmed Ebrahim;
Abstract
Giardia Lamblia is a widely distributed flagellated protozoan parasite that inhabits the small intestine. It is the most prominent human protozoal enteropathogen with a prevalence rate that varies from 2o/o to 5o/o in the industrialized world and 20% to
30o/o in the developing world. Giardiasis is more prevalent in
infancy and children.
Diagnosis of giardiasis is largely dependent on microscopic demonstration of the parasite in the stool, which.yields many false negatives due to intermittent excretion of Giardia Lamblia. Another reliable methods of diagnosis are duodenal aspiration and intestinal biopsy but they are invasive and uncomfortable techniques. So some other diagnostic techniques were introduced and were studied regarding to their value in diagnosis but these studies were not fulfilled up till now especially regarding to their value as methods in cure assessment.
The aim of this work was to evaluate the ELISA for detection of Giardia antigen in the stool, as a diagnostic technique of giardiasis and as a method of cure assessment after treatment.
This study was conducted on 154 children who were chosen from Ain Shams Pediatric Hospital and from Parasitology Department Research Unit. The cases chosen were complaining from gastrointestinal symptoms suggestive of giardiasis. The children were divided into 3 groups, group 1 with giardiasis, group 2 represents infected control group and group 3 represents healthy controls. The patients in group 1 were subdivided into three groups according to intensity of infection (mild, moderate and heavy infection).
30o/o in the developing world. Giardiasis is more prevalent in
infancy and children.
Diagnosis of giardiasis is largely dependent on microscopic demonstration of the parasite in the stool, which.yields many false negatives due to intermittent excretion of Giardia Lamblia. Another reliable methods of diagnosis are duodenal aspiration and intestinal biopsy but they are invasive and uncomfortable techniques. So some other diagnostic techniques were introduced and were studied regarding to their value in diagnosis but these studies were not fulfilled up till now especially regarding to their value as methods in cure assessment.
The aim of this work was to evaluate the ELISA for detection of Giardia antigen in the stool, as a diagnostic technique of giardiasis and as a method of cure assessment after treatment.
This study was conducted on 154 children who were chosen from Ain Shams Pediatric Hospital and from Parasitology Department Research Unit. The cases chosen were complaining from gastrointestinal symptoms suggestive of giardiasis. The children were divided into 3 groups, group 1 with giardiasis, group 2 represents infected control group and group 3 represents healthy controls. The patients in group 1 were subdivided into three groups according to intensity of infection (mild, moderate and heavy infection).
Other data
| Title | ENZYME-LINKED IMMUNO-SORBENT ASSAY VERSUS DIRECT MICROSCOPY IN DIAGNOSIS AND CURE ASSESSMENT OF GIARDIA LAMBLIA INFECTION | Other Titles | دراسة مقارنة بين الفحص الميكروسكوبى المباشر والاليزا فى تشخيص وتقييم العلاج فى حالات الاصابة بطفيل الجيارديا لامبليا | Authors | Sabah Abd Elgany Ahmed Ebrahim | Issue Date | 2001 |
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