Association Of Serum Vitamin D And Cytochrome P450 27b1 Gene Polymorphism In Viral Hepatitis C Egyptian Patients

Abstract

Hepatitis C virus (HCV) is a leading cause of chronic liver disease in the world, making this disease a significant cause of morbidity and mortality. This study aimed to evaluate the interrelation between the vitamin D serum level and the functional polymorphism of 1-alpha hydroxylase (CYP450-27B1) in the response of chronic HCV patients to treatment. The study was conducted in the Medical Research Centre, Faculty of Medicine, Ain Shams University, and included thirty patients who were diagnosed to have chronic hepatitis C and were treated with pegylated interferon (PEG-IFN) and ribavirin combination therapy. 20 healthy subjects were also included as a control group. These patients were divided into two groups responders (10 patients) & non-responders (20 patients).The blood samples from patients and controls were taken for assay of vitamin D level and CYP450-27B1 polymorphism. The results revealed that chronic HCV patients were suffering from deficiency of both 25(OH)-vitamin D and 1, 25 dihydroxyvitamin D (1, 25(OH)2D), but with respect to CYP27B1 genetic polymorphism, no specific genotype for chronic HCV patient. However, responders with a genotype AA of the CYP27B1 had higher serum concentrations of 1, 25(OH)2 D than the responders with genotype AC and CC. In conclusion, HCV-infected patients carrying the homozygous AA of the CYP27B1 have a higher chances to respond to (PEG-IFN)-based treatment. Key Words: Hepatitis C virus, antiviral therapy, pegylated interferon-alpha, Vitamin D deficiency, CYP27B1 genetic polymorphism.