APPLICATION OF NANOPARTICLES FOR SOME PLANT VIRUSES CONTROL

Abstract

The present study aimed to achieve the following objectives: 1. Confirmation of viruses. 2. Preparation and characterization of Chitosan Nanoparticles. 3. Application of nanoparticles in vitro 4. Application of nanoparticles in vivo The results might be summarized as follows:

PART I 1. Confirmation of viruses 1.1 Potato virus Y (PVY) 1.1.1 Confirmation of the Virus Isolate Biologically Symptoms developed on the five host plants (Datura metel, Datura stramonium, Nicotiana tabacum cv.White Burley, N. tabacum cv. Samsun, Nicotiana glutinosa, Solanum lycopersicum and Solanum tuberosum) were used for biological confirmation of potato virus Y. Datura metel showed mottling, vein clearing and crinkle while Datura stramonium showed no symptomes. N. tabacum cv.White Burley showed mosaic, veinal necrosis while N. tabacum cv. Samsun showed severe mosaic, vein clearing and Nicotiana glutinosa showed severe mosaic. Solanum lycopersicum showed mosaic and Solanum tuberosum showed mosaic, necrosis, deformation and leaf narrow 1.1.2. Confirmation of the Virus Isolate Serologically PVY has detected by DAS-ELISA. The positive result was record by ELISA reader and compared with negative control.

1.1.3. Confirmation of the Virus Isolate Molecularly