Immuno Histochemical Study of Ovaian •Neoplasia

Abstract

Summary

The ovary is considered a site of different neoplasms . Various prognostic factors have been established to estimate a patients individual prognosis . These factors include age , stage, Grade , immunohistochemical techniques using various antibodies and flow cytometry . This work aimed at estimating the value of immunohistochemical staining using PCNA/C-erb 2 antibodies as prognostic factors in ovarian neoplasia . The present study included 80 cases of ovarian neoplasia . 60 cases were retrospectively collected from the pathology departement , Faculty of Medicine , Tanta University and 20 cases were prospective . They were divided as follow , 30 cases of serous tumors (10 benign , 10 borderline , 10 malignant ) 25 cases of mucinous tumors ( 10 benign , 10 borderline and 5 well differentiated carciomas). Five cases oendometrioid carcinoma , five cases of ovarian fibroma , ten cases of granulosa cell tumor (2 JGCT & 8 AGCT) and five cases of undifferentiated carcinomas . All formaline fixed paraffin sections were stained with H & E staining for typing and grading . Immunohistochemial procedure using PCNA and C-erb 2 antibodies by avidine biotine method . In epithelial serous and mucinous tumors the mean age for benign group was 32.1 , 28 for the BL group was 40.6 , 41.6 and for the malignant groups was 58.7, 44 respectively. A statistical significat difference in age was detected between BL and Malignant epithelial tumors . Also all Malignant epithelial tumors were post menopausal . Ascitis and peritoneal implants were detected only in malignant epithelial tumors .

According to the FIGO staging system for avarian carcinoma 25 cases were stage Ia (10 SBLT, 4 SMTS, 10 MBLS, 1 MMTS) , 3 cases were stage Ic (all of them were malignant mucinous tumors ) , 5 cases were stage lie (3 SMTS, 2 MMTS, and 2 cases were stage III (both were malignant mucinous).

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