"Study of The Potential Protective Effect of Gonadotropin Releasing Hormone Antagonist on Gamma Radiation Induced Ovarian Failure

Abstract

1. The current study demonstrated that endocrine environment was affected by radiation, as shown by a drastic increase in FSH levels and a significant decrease in AMH and E2 levels. Additionally, as a result of the early depletion of primordial follicles, irradiated females displayed POF. On the other hand, treatment of female rats with cetrorelix corrected the change in AMH, E2, and FSH levels caused by radiation. 2. The number of primordial follicles may have reached such a critical value that the mechanisms controlling recruitment of primordial follicles into the growing pool could not be effective in the irradiated ovaries. As a result, we have demonstrated that whole-body irradiation with a single dose of 3.2 Gy induced a depletion of the growing preantral follicles within a time period of 24 h, with marked reduction in GCs proliferation. In contrast, cetrorelix pre-treatment of the irradiated rats for 10 days preserved ovarian follicles' stock and increased PCNA expression of the irradiated ovaries. 3. Histopathological examination using H&E stain showed that the stromal tissue of the irradiated ovaries occupied most of the ovarian structure which was characterized by severe hemorrhage with very few primordial follicles were detected at the periphery of the ovary. Curiously, treatment of rats with cetrorelix preserved ovarian tissues from radiation-induced hemorrhage and degeneration. 4. Regarding the OS, radiation exposure resulted in a significan