Effect of New Formulation of Nano Calcium Phosphate Based gel on Regeneration of Periapical Lesions after Endodontic Treatment and Periapical Surgery in Dogs

Abstract

The aim of this study was to synthesize and characterize nano Calcium phosphate based gel as a bone graft material: 1) to be used in periapical surgery in the treatment of periapical abscesses, granulomas and cysts, and 2) to be injected through the prepared root canal to reach the periapical area, thus eliminating the need for the aggressive surgical treatment approaches. Furthermore, Klipdent-PL and nano Calcium phosphate based gel were compared in vivo on periradicular regeneration of endodontically treated teeth in dogs in terms of radiographical and histological examination.

Materials and Methods:

β-TCP nanoparticles were prepared based on natural synthesis method. The crystalline structure of NPs was verified by XRD analysis. The appropriate quality of the synthesized material and the ideal synthesis process were confirmed. Also, the average size of the nano β-TCP crystals within CS composite was evaluated using TEM as 8-17 nm. Six adult mongrel dogs were used in this study. The experimented teeth were randomly assigned into three groups (n=63) in which the bony lesions were filled surgically with Klipdent-PL or nano calcium phosphate based gel compar