Phenotypic and Genotypic Detection of Oxacillinases in Carbapenem-Resistant Acinetobacter Species

Abstract

Out of the 50 studied carbapenem-resistant Acinetobacter isolates, 46 (92%) were found to be carbapenemase producers by the MHT, Carbapenem-hydrolyzing oxacillinases (CHLOs) were detected in 45 (90%) of the carbapenem-resistant Acinetobacter isolates by the MHT using the oxacillin (1μg) disc and the DDST using meropenem (10μg) and amoxicillin/clavulanic (20/10μg) discs, the three phenotypic test gave positive results, denoting the presence of carbapenemase of CHLOs type, in 82% of the isolates. PCR revealed that all carbapenem-resistant Acinetobacter isolates carried blaOXA51-like gene (100%). The blaOXA-23-like gene was detected in 78% (39/50), the blaOXA-58-like gene was detected in 74% (37/50), and the blaOXA-24-like gene was detected in 2% (1/50) of the studied isolates. Keywords: Carbapenem resistant Acinetobacter baumannii - Chaperon secretion membrane systems - Imipenem resistant Acinetobacter baumanii