Study of Urinary RNA Profiling as a Potential Biomarker in Type 2 Diabetic Nephropathy Patients

Abstract

Study of Urinary RNA Profiling as a Potential Biomarker in Type 2 Diabetic Nephropathy Patients. Mona Mostafa Abd El-Rahman Ahmed, Biochemistry Department, Faculty of Science, Ain Shams University. Diabetic nephropathy (DN) is the most common cause of end-stage kidney disease worldwide, and is associated with increased morbidity and mortality in patients with both type 1 and type 2 diabetes. Autophagy is a highly conserved ‘self-eating’ pathway by which cells degrade and recycle macromolecules and organelles. Impairment of autophagy is implicated in the pathogenesis of DN. Emerging body of evidence suggests that targeting the autophagic pathway to activate and restore autophagy activity may be renoprotective. Microtubule-associated protein 1 light-chain 3 (LC3) was employed in monitoring the cellular autophagy. Bioinformatic analysis was done to retrieve promising autophagy biomarker relevant to diabetic nephropathy based on previous microarray studies. A total of 65 diabetic patients type II provided a single voided urine samples for quantifying urinary expression of MAP1LC3B RNA by real-time quantitative polymeras.