Bacterial reduction using two different tip diameter in rotation and reciprocation motion (In vitro study)
Rania ElsayedSoliman Hassan;
Abstract
The aim of the present study was to evaluate the effect of tip diameter and file motion on bacterial reduction during cleaning and shaping using One shape (rotary system) sizes (25/0.06), (37/0.06) and Wave one (reciprocating system) sizes(25/0.08), (40/0.08) by the aid of direct count of colony forming units (CFUs).
Sixty single rooted lower premolars freshly extracted human permanent teeth were selected for this study. The teeth were decoronated using a high speed diamond stone with water coolant and the root length was standardized at length of 15mm. Access into the canals was done and working length of the canal was determined visually by inserting a #15 stainless steel k-file until the file tip was visible at the apical foramen under 4X magnification loupe, and the working length (WL) was established 1 mm short of this length. Irrigation was performed using 2.5% NaOCl irrigation, and then was washed with 5mL of saline solution. Then, the teeth were sterilized in autoclave for 15 min at 121 °C. Then the roots were coated with nail polish.
The E. faecalis was inoculated at 37°C for 24 hours and resuspended in saline to reach a final concentration of 3X108 cells/ml, and adjusted to No.1 macFarland turbidity standard. The samples were contaminated with a 3ml of bacterial suspension and fresh bacterial suspension was prepared and replaced every 72-hours for a period of three weeks, then a random specimen from each subgroup were cultured using sterile paper point to confirm the contamination with the bacteria.
Sixty single rooted lower premolars freshly extracted human permanent teeth were selected for this study. The teeth were decoronated using a high speed diamond stone with water coolant and the root length was standardized at length of 15mm. Access into the canals was done and working length of the canal was determined visually by inserting a #15 stainless steel k-file until the file tip was visible at the apical foramen under 4X magnification loupe, and the working length (WL) was established 1 mm short of this length. Irrigation was performed using 2.5% NaOCl irrigation, and then was washed with 5mL of saline solution. Then, the teeth were sterilized in autoclave for 15 min at 121 °C. Then the roots were coated with nail polish.
The E. faecalis was inoculated at 37°C for 24 hours and resuspended in saline to reach a final concentration of 3X108 cells/ml, and adjusted to No.1 macFarland turbidity standard. The samples were contaminated with a 3ml of bacterial suspension and fresh bacterial suspension was prepared and replaced every 72-hours for a period of three weeks, then a random specimen from each subgroup were cultured using sterile paper point to confirm the contamination with the bacteria.
Other data
| Title | Bacterial reduction using two different tip diameter in rotation and reciprocation motion (In vitro study) | Other Titles | التأثير المضاد للبكتيريا باستخدام قطرين مختلفين للحافة وحركتان مختلفتان, حركة دورانيه و حركة ترددية | Authors | Rania ElsayedSoliman Hassan | Issue Date | 2017 |
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