IMPRESSION CYTOLOGY OF THE CHALAZION CONTENTS
David Malak Botros Georgeos;
Abstract
SUMMARY
C
halazion is the most common eyelid swelling.It forms when the Meibomian gland becomes blocked and sebaceous secretions accumulate. Reaction to the material results in granulation tissue and chronic inflammation.
Impression cytology refers to the application of a cellulose acetate filter to the ocular surface to remove the superficial layers of the ocular surface epithelium, stained by different dyes then prepared for light microscopy examination.
The aim of our study is to subject the contents of the chalazia to impression cytology through a team approach of ophthalmologists and pathologists.
Thirty patientsdiagnosed as chalazia with mean age 22.33±10.755 years were enrolled in this study. Chalazia incised and contents subjected to impression cytology technique steps then examined.
This study showed two patterns of inflammation on cytological smears. Twenty four cases recognized as mixed cell granuloma characterized by presence of few neutrophils with a population of plasma cells, lymphocytes, and singly dispersed macrophages, while the other six cases recognized as suppurating granulomawhich resembles the patterns seen in certain forms of tuberculosis but differ in having proteinaceous background, the lipidized nature of the epithelioid and histiocytic granulomas, and the absence of eosinophils.
The study also showed the presence of fibroblasts in 5 cases, macrophages in 23 cases, plasma cells in 22 cases, lymphocytes in 27 cases and neutrophils in 25 cases. We have found in this study higher incidence of lymphocytes and plasma cells in mixed cell granuloma than in suppurating granuloma.
C
halazion is the most common eyelid swelling.It forms when the Meibomian gland becomes blocked and sebaceous secretions accumulate. Reaction to the material results in granulation tissue and chronic inflammation.
Impression cytology refers to the application of a cellulose acetate filter to the ocular surface to remove the superficial layers of the ocular surface epithelium, stained by different dyes then prepared for light microscopy examination.
The aim of our study is to subject the contents of the chalazia to impression cytology through a team approach of ophthalmologists and pathologists.
Thirty patientsdiagnosed as chalazia with mean age 22.33±10.755 years were enrolled in this study. Chalazia incised and contents subjected to impression cytology technique steps then examined.
This study showed two patterns of inflammation on cytological smears. Twenty four cases recognized as mixed cell granuloma characterized by presence of few neutrophils with a population of plasma cells, lymphocytes, and singly dispersed macrophages, while the other six cases recognized as suppurating granulomawhich resembles the patterns seen in certain forms of tuberculosis but differ in having proteinaceous background, the lipidized nature of the epithelioid and histiocytic granulomas, and the absence of eosinophils.
The study also showed the presence of fibroblasts in 5 cases, macrophages in 23 cases, plasma cells in 22 cases, lymphocytes in 27 cases and neutrophils in 25 cases. We have found in this study higher incidence of lymphocytes and plasma cells in mixed cell granuloma than in suppurating granuloma.
Other data
| Title | IMPRESSION CYTOLOGY OF THE CHALAZION CONTENTS | Other Titles | دراسة الخلايا الانطباعيةلمحتويات الأكيــاس الدهنيــة | Authors | David Malak Botros Georgeos | Issue Date | 2015 |
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