The Behavioural Response of Rhodobacter capsulatus B10 and 37b4 to Cadmium and Zinc toxicity
Sanna Mohamed Fahmy;
Abstract
This study is aiming to investigate the effect of Cd2+ (10 and 20 ppm) and Zn2+ (20 and 40 ppm) on growth , photopigments and morphology of R. capsulatus B10 and 37b4. The former strain has a capsule and the latter is capsuless and both strains belong to purple nonsulfur bacteria " Rhodospirillaceae". Particular attention was focused on bioaccumulation, cadmium- and zinc-binding proteins as well as the patterns of protein and lipopolysacchaddes by gel filtration and SDS-PAGE techniques.
The experimental results can be summarized as follows :-
The effect of Cd2
and Zn2
on growth, photopigments and
morphology :
1) Maximum growth of the two strains were reached after 4 days of incubation when they grown in RAH medium phototrophically under anaerobic conditions at 32 °C.
2) As the concentration of both cations increased in the growth medium, the maximum growth rate decreased, the mean generation time prolonged and tolerance index, protein content and bacteriochlorophyll-a content decreased. There were no qualitative changes in the position of absorption maxima of bacteriochlorophyll a and carotenoids, only a decrease in the absorption maxima.
3) Malformation including, filament and minicell are formed m
R.capsulatus 37b4 grown at 20 ppm Cd2+.
The experimental results can be summarized as follows :-
The effect of Cd2
and Zn2
on growth, photopigments and
morphology :
1) Maximum growth of the two strains were reached after 4 days of incubation when they grown in RAH medium phototrophically under anaerobic conditions at 32 °C.
2) As the concentration of both cations increased in the growth medium, the maximum growth rate decreased, the mean generation time prolonged and tolerance index, protein content and bacteriochlorophyll-a content decreased. There were no qualitative changes in the position of absorption maxima of bacteriochlorophyll a and carotenoids, only a decrease in the absorption maxima.
3) Malformation including, filament and minicell are formed m
R.capsulatus 37b4 grown at 20 ppm Cd2+.
Other data
| Title | The Behavioural Response of Rhodobacter capsulatus B10 and 37b4 to Cadmium and Zinc toxicity | Other Titles | طريقة إستجابة بكتريا الرودوباكتر كابسيولاتس بــ 10 و 37 بــ 4 لسمية كل من الكادميوم والزنك | Authors | Sanna Mohamed Fahmy | Issue Date | 2001 |
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