EXPERIMENTAL AND MOLECULAR BIOLOGICAL STUDIES ON THE ROLE OF FLESH FLIES IN THE TRANSMISSION OF TRICHINOSIS

Abstract

The present study is an approach to detect the role of insects as flesh flies in transmission of trichinosis infection from carnivorous animals to another hosts specially herbivorous animals (as sheep, caws, horses and ostrich) and to man, and to establish a fast precised and accurate method as Real-time PCR for detecting the presence of the infection at low level in any tissue in the hosts. Material & methods Infected pig diaphragm muscles were prepared for inducing infection in other hosts. Infection was proved by using trichinoscope and Real-time PCR. A total of 34 rats, were kept in optimum conditions, sacrificed (35 days p.i.) and identified for presence of Trichinella spiralis, then rats were divided into 4 groups: (A), (B), (C) and (D). Group (A), 18 rats feed on infected pig muscles and were sub-divided into 3 groups; Group (A-1), 4 infected rats slaughtered 8 dpi, their intestines were investigated for collection of adult T. spiralis worms, identified by Real-time PCR and considered as the positive control. Group (A-2), 4 infected rats slaughtered 35 dpi, their muscles were investigated microscopically for presence of infection and identified also by Real-time PCR. Group (A-3), 10 infected rats kept as a source of the infection, slaughtered when needed. Group (B), 2 un-infected rats be infected, slaughtered 35 dpi, their muscles used for Induction of infection in Sarcophaga 3rd instar larvae. Group (C), 10 un-infected rats fed on infected Sarcophaga maggots and slaughtered 35 dpi, their muscles were tested for presence of the infection by trichinoscope, and then identified also by Real-time PCR. Group-D, 4 un-infected rats slaughtered, their skeletal muscles, considered as the negative control and examined microscopically then identified also by Real-time PCR. Flesh fly colony of Sarcophaga argyrostoma prepared for proving their role in infection transmission among different hosts.